Abstract
We present a modified method for DNA minipreparation suitable for large-scale screening of transgenic plants. The method is rapid and efficient—one person can prepare DNA from approximately 50 samples per day. The average yield was about 40 μg DNA per 100 mg of fresh tissue, and the A260/A280 was 1.89–2.03. The total DNA extracted by this method could be used for PCR, restriction enzyme digestion, and Southern blotting.
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Lin, RC., Ding, ZS., Li, LB. et al. A rapid and efficient DNA minipreparation suitable for screening transgenic plants. Plant Mol Biol Rep 19, 379 (2001). https://doi.org/10.1007/BF02772839
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DOI: https://doi.org/10.1007/BF02772839