Abstract
Conidia ofBeauveria bassiana were evaluated for activity against adults ofTribolium castaneum, under laboratory conditions closely resembling the stored-grain conditions.
Three doses of the fungus were applied in the treatments: 0.1 g, 0.5 g and 1 g per 20 insects/dose/flask. Treated insects and untreated were incubated in broken wheat. There were 5 replications for each test.
A total of 1600 treated and control insects used were kept in a climatic chamber (70±5% and 27±2°C) for 45 days. Observations were performed daily and the recording of infected cadavers began when the percentage of mortality exceeded 50%. The evaluation of the efficiency of each dose ofB. bassiana was made 14, 21, 28 and 45 days after inoculation.
Mortality of adults that were exposed to the beetles treated with 0.5 g and 1 g of conidia ofB. bassiana / 20 adults was 87 and 85% respectively, within 21 days of exposure. Onset of mortality was further delayed at lower dosage of the fungus; 55% after 21 days. After 28 days there were no difference in mortality between the 0.5 g and 1 g dosages ofB. bassiana. The most efficient treatment was 0.5 g/20 insects, which resulted in a control higher than 50% 14 days post treatment.
Résumé
L’activité des conidies deB. bassiana vis-à-vis d’adultes deTribolium castaneum a été évaluée dans des conditions de laboratoire similaires à celles existant dans des grains stockés.
Les traitements ont consisté en une application de trois doses de champignon: 0,1 g, 0,5 g et 1 g dans des flacons contenant 20 insectes par dose, avec du blé concassé pour les témoins comme pour les individus traités. Les insectes utilisés (au nombre de 1600) ont été placés dans une chambre climatisée (70±5% et 27±2°C) pendant 45 jours. Des observations quotidiennes ont été faites et le contrôle des individus morts infectés a commencé quand le pourcentage de mortalité a dépassé les 50%. L’évaluation de l’efficacité de chaque dose deB. bassiana a été faite 14, 21, 28 et 45 jours après l’inoculation. La mortalité des adultes traités avec 0,5 g et 1 g de conidies deB. bassiana/20 adultes a été respectivement de 87 et 85%, dans les 21 premiers jours d’exposition. Le début de la mortalité a été retardé avec des doses plus faibles du champignon: seulement 55% après 21 jours d’exposition. A partir de 28 jours, il n’y a pas de différence de mortalité observée entre les doses de 0,5 g et de 1 g deB. bassiana. Le traitement le plus efficace a été obtenu avec une dose de 0,5 g / 20 insectes et a produit une mortalité supérieure à 50% après 14 jours.
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Padin, S.B., Dal Bello, G.M. & Vasicek, A.L. Pathogenicity ofBeauveria bassiana for adults ofTribolium castaneum (Col.: Tenebrionidae) in stored grains. BioControl 42, 569–574 (1997). https://doi.org/10.1007/BF02769816
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DOI: https://doi.org/10.1007/BF02769816