Abstract
This study focused on the physiological changes inCryptomeria japonica accompanied by needle color changes during the winter. The physiological measurements include gas exchange, chlorophyll fluorescence, pigments, active oxygen scavenging enzymes, and several proteins in the photosynthetic apparatus. The light-saturated photosynthesis decreased during the winter. Total chlorophyll content and chlorophylla/b ratio decreased; meanwhile xanthophyll cycle pigments and lutein contents increased significantly. These results indicate that cold acclimation had occurred. Photoinhibition was also observed inC. japonica which can be determined from the decreases in photochemical efficiency of PS II and the partial closure of PS II reaction centers. Small and large subunits of Rubisco and LHC II in the needles ofC. japonica showed only a slight decrease during the winter, indicating that photoinhibition inC. japonica probably reflects a protective process to prevent more severe damage to the photosynthetic apparatus during low-temperature stress.
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Adams W. W. III, and Demmig-Adams, B. (1994) Carotenoid composition and down regulation of photosystem II in three conifer species during the winter. Physiol. Plant. 92: 451–458.
Arnon, D. J. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase inBeta vulgaris. Plant Physiol. 24: 1–15.
Asada, K. (1994) Mechanisms for scavenging reactive molecules generated in chloroplasts under light stress.In Photoinhibition of photosynthesis from molecular mechanisms to the field. Baker, N. R. and Bowyer, J. R. (eds.), 471pp, ßios Scientific Publishers, Oxford, 129–142.
Beyer, W. F. and Fridovich, I. (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal. Biochem. 161: 559–566.
Critchley, C. (1988) The chloroplast thylakoid membrance system is a molecular conveyor belt. Photosynth. Res. 19: 265–276.
Demmig, B. and Björkman, O. (1987) Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants. Planta 171: 171–184.
Demmig-Adams, B. (1990) Carotenoids and photoprotection in plants. A role for the xanthophyll zeaxanthin. Biochem. Biophys. Acta 1020: 1–24.
Genty, B., Briantais, J.-M., and Baker, N. R. (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta 990: 87–92.
Gilmore, A. M. and Yamamoto, H. Y. (1991) Resolution of lutein and zeaxanthin using a non-endcapped, lightly carbon-loaded C18 high-performance liquid chromatographic column. J. Chromatogr. 543: 137–145.
Ida, K. (1981) Eco-physiological studies on the response of taxodiaceous conifers to shading, with special reference to the behaviour of leaf pigments. 1. Distribution of carotenoids in green and autumnal reddish-brown leaves of gymnosperms. Bot. Mag. 94: 41–54.
Kitajima, M. and Butler, W. L. (1975) Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromoquinone. Biochim. Biophys. Acta 376: 105–115.
Krause, G. H. (1988) Photoinhibition of photosynthesis. An evaluation of damaging and protective mechanisms. Physiol. Plant 74: 566–574.
Krivosheeva, A., Tao, D., Ottander, C., Wingsle, G., Dube, S., and Öquist, G. (1996) Cold acclimation and photoinhibition of photosynthesis in Scots pine. Planta 200: 296–305.
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
Lovelock, C. E. and Winter, K. (1996) Oxygen-dependent electron transport and protection from photoinhibition in leaves of tropical tree species. Planta 198: 580–587.
Nakano, Y. and Asada, K. (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22: 867–880.
Ögren, E. and Öquist, G. (1984) Photoinhibition of photosynthesis inLemna gibba as induced by the interaction between light and temperature. III. Chlorophyll fluorescence at 77K. Physiol. Plant 62: 193–200.
Osmond, C. B. (1994) What is photoinhibition? Some insights from comparisons of shade and sun plants.In Photoinhibition of photosynthesis from molecular mechanisms to the field. Baker, N. R. and Bowyer, J. R. (eds.), 471pp., ßios Scientific Publishers, Oxford, 1–24.
Osmond, C. B. and Grace, S. C. (1995) Perspectives on photoinhibition and photorespiration in the field: quintessential inefficiencies of the light and dark reactions of photosynthesis? J. Exp. Bot. 46: 1351–1362.
Ottander, C. and Öquist, G. (1991) Recovery of photosynthesis in winter-stressed Scots pine. Plant Cell Environ. 14: 345–349.
Ottander, C., Campbell, D., and Öquist, G. (1995) Seasonal changes in photosystem II organisation and pigment composition inPinus sylvestris. Planta 197:176–183.
Oxborough, K. and Baker, N. R. (1997) Resolving chlorophylla fluorescence images of photosynthetic efficiency into photochemical and non-photochemical components-calculation ofqP andF v′/F m′ without measuringF 0′. Photosynth. Res. 54: 135–142.
Peterson, G. L. (1977) Review of folin phenol protein quantiation method of Lowry, Rosebrough, Farr and Randell. Anal. Biochem. 100: 201–220.
Powles, S. B. (1984) Photoinhibition of photosynthesis induced by visible light. Annu. Rev. Plant Physiol. 35: 15–44.
Prioul, J. L. and Chartier, P. (1977) Partitioning of transfer and carboxylation components of intracellular resistance to photosynthetic CO2 fixation: A critical analysis of the methods used. Ann. Bot. 41: 789–800.
Strand, M. and Öquist, G. (1985) Inhibition of photosynthesis by freezing temperatures and high light levels in cold-acclimated seedlings of Scots pine (Pinus sylvestris). I. Effects on the light-limited and light-saturated rates of CO2 assimilation. Physiol. Plant. 64: 425–430.
Telfer, A. and Barber, J. (1994) Elucidating the molecular mechanisms of photoinhibition by studying isolated photosystem II reaction centres.In Photoinhibition of photosynthesis from molecular mechanisms to the field. Baker, N. R. and Bowyer, J. R. (eds.), 471pp, ßios Scientific Publishers, Oxford, 25–50.
Weger, H. G., Silim, S. N., and Guy, R. D. (1993) Photosynthetic acclimation to low temperature by western red cedar seedlings. Plant Cell Environ. 16: 711–717.
Wu, J., Neimanis, S., and Heber, U. (1991) Photorespiration is more effective than the mehler reaction in protecting the photosynthetic apparatus against photoinhibition. Bot. Acta 104: 283–291.
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This research was supported by the Bio-oriented Technology Research Advancement Institute (BRAIN), Japan
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Han, Q., Mukai, Y. Cold acclimation and photoinhibition of photosynthesis accompanied by needle color changes inCryptomeria japonica during the winter. J For Res 4, 229–234 (1999). https://doi.org/10.1007/BF02762253
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DOI: https://doi.org/10.1007/BF02762253