Abstract
Langitudinally sliced embryonic axes from pea and lentil mature seeds cocultivated withA. tumefaciens carrying agus reporter gene in its T-DNA provided a convenient means to evaluate the efficiency of gene transfer to tissues in different cultivars and cocultivation conditions. Use of this technique demonstrated wide variation in susceptibility toAgrobacterium among several pea and lentil commercial genotypes.
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Lurquin, P.F., Cai, Z., Stiff, C.M. et al. Half-embryo cocultivation technique for estimating the susceptibility of pea (Pisum sativum L.) and lentil (Lens culinaris Medik.) cultivars toAgrobacterium tumefaciens . Mol Biotechnol 9, 175–179 (1998). https://doi.org/10.1007/BF02760819
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DOI: https://doi.org/10.1007/BF02760819