Abstract
Growth-phase associated changes in and relationships between the specific growth rate (μ) characterizing the reproductive capacity of the cells, the contents of intracellular biogenic polyamines (BPA), such as putrescine (P), cadaverine (C), and spermidine (S), and antilysozyme activity (ALA) were studied in 37 strains ofEscherichia coli grown in batch culture on solid medium. A decrease in μ upon the transition of the culture to the stationary growth phase was accompanied by a decrease in the pool of free BPA, mainly P and C, and by the appearance of ALA. The interrelations between the parameters studied were described as a complex of direct and negative correlations; the combination of low initial P and C contents, reduced P/S and C/S ratios, and a high level of ALA was designated as afactor of slight inhibition ofE. coli reproduction. It is argued that BPA and ALA are integrated in a system controlling both the metabolism and stability of peptidoglycan inE. coli.
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Bukharin, O.V., Gritsenko, V.A., Tkachenko, A.G. et al. Growth-phase associated changes in the reproductive capacity, intracellular polyamines, and antilysozyme activity inEscherichia coli batch culture. Microbiology 69, 113–115 (2000). https://doi.org/10.1007/BF02757267
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DOI: https://doi.org/10.1007/BF02757267