Abstract
For much of the last hundred years most cases of amoebiasis have been diagnosed by light microscopy. Only relatively recently have we become aware that this technique is usually incapable of distinguishing between two species-Entamoeba histolytica andE. dispar-only the first of which is a pathogen. The implications of this for patient management and, even more, for the validity of epidemiological surveys, are only slowly being addressed. What is clear is that methods are urgently required to distinguish between infections with these two species and this review attempts to summarise some of those, which have been developed to meet this need.
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Abd-Alla M D, Jackson T F H G, Gathiram V, el Hawey A M and Ravdin J I 1993 Differentiation of pathogenicEntamoeba histolytica infections from nonpathogenic infections by detection of galactose-inhibitable adherence protein antigen in sera and feces;J. Clin. Microbiol. 31 2845–2850
Abd-Alla M D, Jackson T G and Ravdin J I 1998 Serum IgM antibody response to the galactose-inhibitable adherence lectin ofEntameoba histolytica;Am. J. Trop. Med. Hyg. 59 431–434
Abd-Alla M D, Jackson T F, Reddy S and Ravdin J I 2000a Diagnosis of invasive amebiasis by enzyme-linked immunosorbent assay of saliva to detect amoebic lectin antigen and anti-lectin immunoglobulin G antibodies;J. Clin. Microbiol. 38 2344–2347
Abd-Alla M D, Wahib A A and Ravdin J I 2000b Comparison of antigen-capture ELISA to stool-culture methods for the detection of asymptomatic entamoeba species infection in Kafer Daoud, Egypt;Am. J. Trop. Med. Hyg. 62 579–582
Abd-Alla M D and Ravdin J I 2002 Diagnosis of amoebic colitis by antigen capture ELISA in patients presenting with acute diarrhoea in Cairo, Egypt;Trop. Med. Int. Health 7 365–370
Acuna-Soto R, Samuelson J, De Girolami P, Zarate L, Millan Velasco F, Schoolnick G and Wirth D 1993 Application of the polymerase chain reaction to the epidemiology of pathogenic and nonpathogenicEntamoeba histolytica;Am. J. Trop. Med. Hyg. 48 58–70
Agarwal S K, Guleria P, Gupta S, Goel V and Bhattacharya S 1998 Role of DNA probes in characterization of pathogenic and non-pathogenicE. histolytica;J. Assoc. Phys. India 46 701–703
Bhattacharya A, Arya R, Clark C G and Ackers J P 2000 Absence of lipophosphoglycan-like glycoconjugates inEntamoeba dispar;Parasitology 120 31–35
Blanc D and Sargeaunt P G 1991Entamoeba histolytica zymodemes: exhibition of gamma and delta bands only of glucose phosphate isomerase and phosphoglucomutase may be influenced by starch content in the medium;Exp. Parasitol. 72 87–90
Britten D, Wilson S M, McNerney R, Moody A H, Chiodini P L and Ackers J P 1997 An improved colorimetric PCR-based method for detection and differentiation ofEntamoeba histolytica andEntamoeba dispar in feces;J. Clin. Microbiol. 35 1108–1111
Bruchhaus I, Jacobs T, Leippe M and Tannich E 1996Entamoeba histolytica andEntamoeba dispar. differences in numbers and expression of cysteine proteinase genes;Mol. Microbiol. 22 255–263
Brumpt E 1925 Etude sommaire de l’ “Entamoebadispar” n.sp. Amibe a kystes quadrinuclees, parasite de l’ homme;Bull. Acad. Med. (Paris) 94 943–952
Clark C G 1995 Axenic cultivation ofEntamoeba dispar Brumpt 1925,Entamoeba insolita Geiman and Wichterman 1937 andEntamoeba ranarum Grassi 1879;J. Euk. Microbiol. 42 590–593
Clark C G and Diamond L S 1991 The Laredo strain and other’Entamoeba histolytica-like’ amoebae areEntamoeba moshkovskii;Mol. Biochem. Parasitol. 46 11–18
Clark C G, Espinosa-Cantellano M and Bhattacharya A 2000Entamoeba histolytica: an overview of the biology of the organism; inAmebiasis (ed.) J I Ravdin(London: Imperial College Press) 1st edition, pp 1–45
Diamond L S and Clark C G 1993 A redescription ofEntamoeba histolytica Schaudinn 1903 (Emended Walker 1911) separating it fromEntamoeba dispar Brumpt 1925;J. Euk. Microbiol. 40 340–344
Evangelopoulos A, Legakis N and Vakalis N 2001 Microscopy, PCR and ELISA applied to the epidemiology of amoebiasis in Greece;Parasitol. Int. 50 185–189
Evangelopoulos A, Spanakos G, Patsoula E, Vakalis N and Legakis N 2000 A nested, multiplex, PCR assay for the simultaneous detection and differentiation ofEntamoeba histolytica andEntamoeba dispar in faeces;Ann. Trop. Med. Parasitol. 94 233–240
Garcia L S and Bruckner D A 1997Diagnostic medical parasitology 3rd edition (Washington DC: ASM Press)
Gathiram V and Jackson T F H G 1987 A longitudinal study of asymptomatic carriers of pathogenic zymodemes ofEntamoeba histolytica;S. Afr. Med. J. 72 669–672
Gilman R, Islam M, Paschi S, Goleburn J and Ahmad F 1980 Comparison of conventional and immunofluorescent techniques for the detection ofEntamoeba histolytica in rectal biopsies;Gastroenterology 78 435–439
González-Ruíz A, Haque R, Aguirre A, Castanon G, Hall A, Guhl F, Ruiz-Palacios G, Miles M A and Warhurst D C 1994 Value of microscopy in the diagnosis of dysentery associated with invasiveEntamoeba histolytica;J. Clin. Pathol. 47 236–239
Haque R, Ali I K, Akther S and Petri W A Jr 1998 Comparison of PCR, isoenzyme analysis, and antigen detection for diagnosis ofEntamoeba histolytica infection;J. Clin. Microbiol. 36 449–452
Haque R, Ali I M and Petri W A Jr 1999 Prevalence and immune response toEntamoeba histolytica infection in preschool children in Bangladesh;Am. J. Trop. Med. Hyg. 60 1031–1034
Haque R, Ali I M, Sack R B, Farr B M, Ramakrishnan G and Petri W A Jr 2001 Amebiasis and Mucosal IgA Antibody against theEntamoeba histolytica Adherence Lectin in Bangladeshi Children;J. Infect. Dis. 183 1787–1793
Haque R, Mollah N U, Ali I K, Alam K, Eubanks A, Lyerly D and Petri W A Jr 2000 Diagnosis of amoebic liver abscess and intestinal infection with the TechLabEntamoeba histolytica II antigen detection and antibody tests;J. Clin. Microbiol. 38 3235–3239
Hossain M M, Ljungstrom I, Glass R I, Lundin L, Stoll B J and Huldt G 1983 Amoebiasis and giardiasis in Bangladesh: parasitological and serological studies;Trans. R. Soc. Trop. Med. Hyg. 77 552–554
Hughes M A and Petri W A Jr 2000 Amoebic liver abscess;Infect. Dis. Clin. North. Am. 14 565–582, viii
Jackson T F H G, Gathiram V and Simjee A E 1985 Seroepidemiological study of antibody responses to the zymodemes ofEntamoeba histolytica;Lancet 1 716–719
Katzwinkel-Wladarsch S, Loscher T and Rinder H 1994 Direct amplification and differentiation of pathogenic and nonpathogenicEntamoeba histolytica DNA from stool specimens;Am. J. Trop. Med. Hyg. 51 115–118
Kean B H, Mott K E and Russell A J 1978 Lösch F A Massive development of amoebae in the large intestine (Massenhafte Entwickelung von Amöben im Dickdarm) (Translation); inTropical medicine and parasitology, classic investigations (New York: Cornell University Press) vol. 1, pp. 71–79
Lotter H, Jackson T F and Tannich E 1995 Evaluation of three serological tests for the detection of antiamoebic antibodies applied to sera of patients from an area endemic for amebiasis;Trop. Med. Parasitol. 46 180–182
Martínez-Palomo A, González-Robles A and de la Torre M 1973 Selective agglutination of pathogenic strains ofEntamoeba histolytica induced by Con A;Nat. New Biol. 245 186–187
Mirelman D, Nuchamowitz Y and Stolarsky T 1997 Comparison of use of enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes for simultaneous detection ofEntamoeba histolytica andE. dispar;J. Clin. Microbiol. 35 2405–2407
Ong S J, Cheng M Y, Liu K H and Horng C B 1996 Use of the ProSpecT microplate enzyme immunoassay for the detection of pathogenic and non-pathogenicEntamoeba histolytica in faecal specimens;Trans. R. Soc. Trop. Med. Hyg. 90 248–249
Petri W A Jr, Haque R, Lyerly D and Vines R R 2000 Estimating the impact of amebiasis on health;Parasitol. Today 16 320–321
Petri W A Jr, Jackson T F H G, Gathiram V, Kress K, Saffer L D, Snodgrass T L, Chapman M D, Keren Z and Mirelman D 1990a Pathogenic and nonpathogenic strains ofEntamoeba histolytica can be differentiated by monoclonal antibodies to the galactosespecific adherence lectin;Infect. Immun. 58 1802–1806
Petri W A Jr and Singh U 1999 Diagnosis and management of amebiasis;Clin. Infect. Dis. 29 1117–1125
Petri W A Jr, Snodgrass T L, Jackson T F H G, Gathiram V, Simjee A E, Chadee K and Chapman M D 1990b Monoclonal antibodies directed against the galactose-binding lectin ofEntamoeba histolytica enhance adherence;J. Immunol. 144 4803–4809
Pillai D R, Keystone J S, Sheppard D C, MacLean J D, MacPherson D W and Kain K C 1999Entamoeba histolytica andEntamoeba dispar: epidemiology and comparison of diagnostic methods in a setting of nonendemicity;Clin. Infect. Dis. 29 1315–1318
Ravdin J I and Petri W A Jr 1994Entamoeba histolytica (Amebiasis); inPrinciples and practice of infectious diseases, 4th edition (eds) G L Mandell, J E Bennett and R Dolin (New York: Churchill Livingstone) pp 2395–2408
Rivera W L, Tachibana H, Silva Tahat M R, Uemura H and Kanbara H 1996 Differentiation ofEntamoeba histolytica andE. dispar DNA from cysts present in stool specimens by polymerase chain reaction: its field application in the Philippines;Parasitol. Res. 82 585–589
Samuelson J, Acuna-Soto R, Reed S, Mirelman D, Schoolnik G, Zarate L and Wirth D 1989 DNA probe for diagnosis ofEntamoeba histolytica;Abstracts, XI Seminaro Sobre Amibiasis, Mexico p. 40
Sargeaunt P G 1988 Zymodemes ofEntamoeba histolytica; inAmebiasis: Human infection by Entamoeba histolytica (ed.) J I Ravdin (New York: John Wiley) 1st edition, pp 370–387
Schunk M, Jelinek T, Wetzel K and Nothdurft H D 2001 Detection ofGiardia lamblia andEntamoeba histolytica in stool samples by two enzyme immunoassays;Eur. J. Clin. Microbiol. Infect. Dis. 20 389–391
Sehgal R, Abd-Alla M, Moody A H, Chiodini P L and Ackers J P 1995 Comparison of two media for the isolation and shortterm culture ofEntamoeba histolytica andE. dispar;Trans. R. Soc. Trop. Med. Hyg. 89 394
Shetty N, Das P, Pal S C and Prabhu T 1988 Observations on the interpretation of amoebic serology in endemic areas;J. Trop. Med. Hyg. 91 222–227
Stanley S L Jr, Jackson T F, Foster L and Singh S 1998 Longitudinal study of the antibody response to recombinantEntamoeba histolytica antigens in patients with amoebic liver abscess;Am. J. Trop. Med. Hyg. 58 414–416
Strachan W D, Chiodini P L, Spice W M, Moody A H and Ackers J P 1988 Immunological differentiation of pathogenic and non-pathogenic isolates ofEntamoeba histolytica;Lancet 1 561–563
Tachibana H, Kobayashi S, Okuzawa E and Masuda G 1992 Detection of pathogenicEntamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction;Int. J. Parasitol. 22 1193–1196
Tannich E and Burchard G D 1991 Differentiation of pathogenic from non-pathogenicEntamoeba histolytica by restriction fragment analysis of a single gene amplifiedin vitro;J. Clin. Microbiol. 29 250–255
Troll H, Marti H and Weiss N 1997 Simple differential detection ofEntamoeba histolytica andEntamoeba dispar in fresh stool specimens by sodium acetate-acetic acid-formalin concentration and PCR;J. Clin. Microbiol. 35 1701–1705
Verweij J J, Blotkamp J, Brienen E A, Aguirre A and Polderman A M 2000 Differentiation ofEntamoeba histolytica andEntamoeba dispar cysts using polymerase chain reaction on DNA isolated from faeces with spin columns;Eur. J. Clin. Microbiol. Infect. Dis. 19 358–361
Walker E L and Sellards W S 1913 Experimental entamoebic dysentery;Phillipine J. Sci. B. Trop. Med. 8 253–331
Walsh J A 1986 Problems in recognition and diagnosis of amebiasis estimation of the global magnitude of morbidity and mortality;Rev. Infect. Dis. 8 228–238
WHO 1969 Amoebiasis;WHO Tech. Rep. Ser. No. 421
WHO 1997Entamoeba taxonomy;Bull. WHO 75 291–292
Willhoeft U, Buss H and Tannich E 1999a DNA sequences corresponding to the ariel gene family ofEntamoeba histolytica are not present inE. dispar;Parasitol. Res. 85 787–789
Willhoeft U, Hamann L and Tannich E 1999b A DNA sequence corresponding to the gene encoding cysteine proteinase 5 inEntamoeba histolytica is present and positionally conserved but highly degenerated inEntamoeba dispar;Infect. Immun. 67 5925–5929
Zaman S, Khoo J, Ng S W, Ahmed R, Khan M A, Hussain R and Zaman V 2000 Direct amplification ofEntamoeba histolytica DNA from amoebic liver abscess pus using polymerase chain reaction;Parasitol. Res. 86 724–728
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Packers, J. The diagnostic implications of the separation ofEntamoeba histolytica andEntamoeba dispar . J Biosci 27, 573–578 (2002). https://doi.org/10.1007/BF02704850
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DOI: https://doi.org/10.1007/BF02704850