Abstract
Factors affecting the quantitative analysis of triglycerides from natural oils were investigated on reverse-phase HPLC columns. The organic solvent used to dissolve the triglycerides greatly determined the extent to which the solute molecules interacted with the mobile phase and hydrocarbonaceous ligand. Chloroform-dissolved triglycerides resulted in better resolution and detection of the solute matrix of all the organic solvents used. Also, ethanol was superior to methanol as the polar organic modifier in the mobile-phase composition. Ballistic temperature programming significantly reduced analysis time and column temperature, when raised high enough, can reverse the elution of some solutes, Possible mechanisms of triglyceride solute retention on reverse-phase columns are advanced in conjunction with the various parameters used in the investigation.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Lochmuller, C.H., and R.R. Wilder, J. Chromatogr. Sci. 17:574 (1979).
Horvath, C., and W. Melander, Chromatogr. 11:262 (1978).
Parris, N.A., J. Chromatogr. 157:162 (1978).
Plattner, R.D., G.F. Spencer, and R. Kleiman, JAOCS 54:511 (1977).
Scott, R.P.W., and P. Kucera, J. Chromatogr. 171:51 (1979)
Synder, L.R., and J.J. Kirland, Modern Practice of Liquid Chromatography, Wiley-InterScience, New York, 1971, p. 207.
Gilpin, R.K., and J.A. Squires, J. Chromatogr. Sci. 19:195 (1981).
Sisco, W.R., and R.K. Gilpin, Ibid. 19:4 (1980).
Author information
Authors and Affiliations
Additional information
Paper Number 8705 of the Journal Series of the NC Agr. Res. Serv. (NCARS).
About this article
Cite this article
Singleton, J.A., Pattee, H.W. Optimization of parameters for the analysis of triglyceride by reverse phase HPLC using a UV detector at 210 nm. J Am Oil Chem Soc 61, 761–766 (1984). https://doi.org/10.1007/BF02672130
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02672130