Abstract
Grape berries contain compounds that aggregate with and precipitate RNA in the presence of chaotropic agents or phenol. The procedure described here extracts RNA from finely ground tissues using mild denaturants, and selectively precipitates the aggregate-forming material with 30% ethanol. The resulting RNA is suitable for northern blot analysis and translationin vitro.
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Abbreviations
- ATA:
-
aurintricarboxylic acid
- DEPC:
-
diethyl pyrocarbonate
- DTT:
-
dithiothreitol
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Tesniere, C., Vayda, M.E. Method for the isolation of high-quality RNA from grape berry tissues without contaminating tannins or carbohydrates. Plant Mol Biol Rep 9, 242–251 (1991). https://doi.org/10.1007/BF02672074
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DOI: https://doi.org/10.1007/BF02672074