Abstract
Hairy roots, incited byAgrobacterium rhizogenes, form a useful system for analysing the expression and phenotypic effects of foreign genes in plant root tissue. Image analysis offers a non-invasive method of describing their growth in culture. Images of pea (coarse) andBrassica (fine) hairy roots were captured, processed and analysed without difficulty using a commercially available image analysis system. The value of this method in monitoring intermediate changes in growth pattern was illustrated by following the changes in five putatively chlorsulfuron-resistantBrassicc hairy-root lines cultured with and without a selective level of chlorsulfuron. Areas of hairy-root research where this technique will be particularly useful are discussed.
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Abbreviations
- LS medium:
-
Linsmaier and Skoog medium
- T-DNA:
-
transferred DNA
- TL :
-
left transferred DNA
- TR :
-
right transferred DNA
- d.f.:
-
degrees of freedom
- Ti :
-
tumor-inducing plasmid
- Ri :
-
hairy-root-inducing plasmid
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Coles, G.D., Abernethy, D.J., Christey, M.C. et al. Monitoring hairy-root growth by image analysis. Plant Mol Biol Rep 9, 13–20 (1991). https://doi.org/10.1007/BF02669285
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DOI: https://doi.org/10.1007/BF02669285