Abstract
Maximum specific growth rate (μmax) ofPhaeodactylum tricornutum increased with increasing culture reactor surface-to-volume ratio. Values for μmax of 0.647, 0.377 and 0.339 day−1 were observed for the 75-mL tube, 5.6-L tank and the 16-L tank, respectively. Higher biomass was achieved in the 75-mL batch culture tube under continuous light as compared with light cycle conditions. Palmitic acid, palmitoleic acid and eicosapentaenoic acid (EPA) accounted for over 60% of total fatty acids in the batch tube culture, with EPA content increasing to a maximum after three days. In chemostat cultures, run at dilution rates of 0.15 day−1 (0.45 of μmax) and 0.3 day−1 (0.9 of μmax), cell concentration reached a steady state of 2.18 and 0.7 g/L, respectively, while contents of EPA per liter of culture at steady state were 100.9 and 82.5 mg/L, respectively. At both dilution rates, EPA content of total fatty acids was the same (35.0–35.2%). At a dilution rate of 0.3 day−1, the continuous culture system manifested productivities of 0.51 g/L/d and 25.1 mg/L/d for biomass and EPA, respectively.
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Yongmanltchal, W., Ward, O.P. Growth and eicosapentaenoic acid production byPhaeodactylum tricornutum in batch and continuous culture systems. J Am Oil Chem Soc 69, 584–590 (1992). https://doi.org/10.1007/BF02636113
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DOI: https://doi.org/10.1007/BF02636113