Summary
Cell suspension cultures of buffelgrass were established from two types of callus, a friable tan callus and a brown gelatinous callus, using Murashige and Skoog medium containing 13.6 µM 2,4-dichlorophenoxyacetic acid (2,4-D). The friable callus formed a rapidly growing suspension culture, designated BG, which had a doubling time of 2.5 days. The gelatinous callus formed a very slow-growing suspension culture, designated BGG, which had a doubling time of 1 mo. During growth, the medium of the BGG line slowly increased in viscosity, becoming a thickened gel by the end of the subculture period. Both lines had high cell viability. Embryogenesis could be induced in both lines by culturing on charcoal-containing, 2,4-D-free medium. No embryos formed in the absence of charcoal.
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Dethier Rogers, S.M., Dahmer, M.L. & Stair, D.W. Characterization of buffelgrass (Cenchrus Ciliaris L.) cell suspension cultures. In Vitro Cell Dev Biol - Plant 29, 51–54 (1993). https://doi.org/10.1007/BF02632251
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DOI: https://doi.org/10.1007/BF02632251