Abstract
The genetic polymorphism of Malagasy prosimian populations is studied by using RAPD markers. The analysis includes twoLepilemur septentrionalis populations of the area of Analamera separated by a deforested hill crest and one population ofL. dorsalis from the island of Nosy-Be. The genetic diversity is higher in the two populations of Analamera than in that of Nosy-Be and the level of genetic differentiation is higher between the population ofL. dorsalis and the two populations ofL. septentrionalis than between the two populations ofL. septentrionalis themselves. Despite the hill crest separating the two populations ofL. septentrionalis, our results demonstrate that they belong to one population. The respective roles of the geographical barriers and the reproductive barrier between the two species, are discussed.
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Bardakci, F.;Skibinski, D. O. F. 1994. Application of the RAPD technique in tilapia fish: species and subspecies identification.Heredity, 73: 117–123.
Battistini, M. 1960. (cited byPaulian, R. 1961) La Zoogéographie de Madagascar et des îles voisines. In:Faune de Madagascar, Vol. 13,Paulian,R. (ed.), Publ. I.R.S.M., Tananarive, p. 67.
Clark, A. G.;Lanigan, C. M. S. 1993. Prospects for estimating nucleotide divergence with RAPDs.Mol. Biol. Evol., 10: 1096–1111.
Darga, L. L.;Goodman, M.;Weiss, M. L.;Moore, G. W.;Prychodk, W.;Dene, H.;Tashian, R.;Koen, A. A. 1975. Molecular systematics and clinal variation in macaques. In:Isoenzymes: IV-Genetics and Evolution,Markert,C. L. (ed.), Academic Press, New York, pp. 797–818.
Gibbs, H. L.;Prior, K. A.;Weatherhead, P. J. 1994. Genetic analysis of populations of threatened snake species using RAPD markers.Mol. Ecol., 3: 329–337.
Giese, H.;Holm-Jensen, A. G.;Mathiassen, H.;Kjaer, B.;Rasmussen, S. K.;Bay, H. 1994. Distribution of RAPD markers on a linkage map of barley.Hereditas, 120: 267–273.
Harada, T.;Matsukawa, K.;Sato, T.;Ishikawa, R.;Niizeki, M.;Saito, K. 1993. DNA-RAPDs detect genetic variation and paternity inMalus.Euphytica, 65: 87–91.
Hartl, D. L. 1988.A Primer of Population Genetics. Sinauer Associates, Sunderland, Massachusetts.
Ishak, B.;Warter, S.;Dutrillaux, B.;Rumpler, Y. 1992. Chromosomal rearrangements and speciation of sportive lemurs (Lepilemur species).Folia Primatol., 58: 121–130.
Ishimoto, G. 1973. Blood protein variations in Asian macaques: III. Characteristics of the macaque blood protein polymorphism.J. Anthropol. Soc. Nip., 81: 1–13.
Leipoldt, M.;Schiltz, A.;Ganzhorn, J. H.;Tomiuk, J. 1996. Genetic analysis ofLepilemur mustelinus ruficaudatus populations using RAPD markers. In:Biogéographie de Madagascar,Lourençon,W. (ed.), O.R.S.T.O.M., Paris, pp. 267–274.
Lynch, M.;Milligan, B. G. 1994. Analysis of population genetic structure with RAPD markers.Mol. Ecol., 3: 91–99.
Maki, M.;Horie, S. 1999. Random amplified polymorphic DNA (RAPD) markers reveal less genetic variation in the endangered plantCerastium fischerianum var.molle than in the widespread conspecificC. fischerianum var.fischerianum (Caryophyllaceae).Mol. Ecol., 8: 145–150.
Micheli, M. R.;Bova, E.;Pascal, E.;D’Ambrisio, E. 1994. DNA fingerprinting with the random amplified DNA (RAPD) method.Nucl. Acids Res., 22: 1921–1922.
Neveu, H.;Haffn, T.;Zimmermann, E.;Rumpler, Y. 1998. Comparison of the genetic diversity of wild and captive groups ofMicrocebus murinus using the random amplified polymorphic DNA method.Folia Primatol., 69: 124–135.
Park, Y. H.;Kohel, R. J. 1994. Effect of concentration of MgCl2 on random-amplified DNA polymorphism.Biotechniques, 16: 652–655.
Powell, W.;Morgante, M.;Andre, C. 1996. The comparison of RFLP, RAPD, AFLP, and SSR (microsatellite) markers for germplasm analysis.Mol. Breed., 2: 225–238.
Rabarivola, C.;Meier, B.;Langer, C.;Scheffrahn, W.;Rumpler, Y. 1996. Population genetics ofEulemur macaco macaco (Primates: Lemuridae) on the islands of Nosy-Be and Nosy-Komba and the Peninsula of Ambato (Madagascar).Primates, 37: 215–225.
Ratomponirina, C.;Brun, B.;Rumpler, Y. 1988. Synaptonemal complexes in Robertsonian translocation heterozygous in lemurs. In:Kew Chromosomes,Brandham,P. E. (ed.), HMSO Publ. Centre, London, pp. 65–73.
Rieseberg, L. H. 1996. Homology among RAPD fragments in interspecific comparison.Mol. Ecol., 5: 99–105.
Rumpler, Y. 2000. What cytogenetic studies may tell us about species diversity and speciation of lemurs.Int. J. Primatol., 21: 865–881.
Rumpler, Y.;Dutrillaux, B. 1990.Chromosomal Evolution and Speciation in Primates: Revisiones sobre Biologia Cellular, Vol. 23. Univ. of the Basque Country (ed.), Springer-Verlag, Berlin/Heidelberg/New York.
Sambrook, J.;Fritsch, E. F.;Maniatis, T. 1990.Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York.
Williams, J. G. K.;Kubelik, A. R.;Livak, K. J.;Rafaiski, J. A.;Tingey, S. V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers.Nucl. Acids Res., 18: 6531–6535.
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Ravaoarimanana, B., Fausser, JL. & Rumpler, Y. Genetic comparison of wild populations ofLepilemur septentrionalis andLepilemur dorsalis using RAPD markers. Primates 42, 221–231 (2001). https://doi.org/10.1007/BF02629638
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DOI: https://doi.org/10.1007/BF02629638