EDITOR'S STATEMENT This paper documents the interesting observation that glandular cells from anterior pituitary contain laminin in their basement membranes and also apparently synthesize and secrete this extracellular matrix component. Gordon H. Sato
Summary
The distribution of laminin was investigated by immunocytochemistry in the rat anterior pituitary in vivo and in primary culture. It was localized by immunofluorescence and by immunoperoxidase in the basement membranes of the pituitary in vivo. In addition it was also found inside glandular cells both in vivo and in culture. The number of immunoreactive cells greatly varied depending on the technical approach used. It was always higher in primary cultures than in vivo. At the electron microscope level, a staining was observed on secretory granules, on rough endoplasmic reticulum cisternae as well as on the membrane of some Golgi saccules and vesicles. Such a localization, at the level of subcellular sites involved in the secretory process, suggests that these cells are able to synthesize and to export in vivo as well as in vitro this component of their basement membranes.
Article PDF
Avoid common mistakes on your manuscript.
References
Alitalo, K.; Kurkinen, M.; Vaheri, A.; Virtanen, I.; Rohde, H.; Timpl, R. Basal lamina glycoproteins are produced by neuroblastoma cells. Nature 287: 465–466; 1980.
Ash, J. F.; Louvard, D.; Singer, S. J. Antibody-induced linkages of plasma membrane proteins to intracellular actomyosin-containing filaments in cultured fibroblasts. Proc. Natl. Acad. Sci. USA 74: 5584–5588; 1977.
Chung, A. E.; Jaffe, R.; Freeman, L. L.; Vergnes, J. P.; Braginski, J. E.; Carlin, B. Properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma derived cell line. Cell 16: 277–287; 1979.
Courtoy, P. J.; Timpl, R.; Farquhar, M. G. Comparative distribution of laminin, type IV collagen, and fibronectin in the rat glomerulus. J. Histochem. Cytochem. 30: 874–886; 1982.
Courtoy, P. J.; Picton, D. H.; Farquhar, M. G. Resolution and limitations of the immunoperoxidase procedure in the localization of extracellular matrix antigens. J. Histochem. Cytochem. 31: 945–951; 1983.
Faivre-Bauman, A.; Puymirat, J.; Loudes, C.; Barret, A.; Tixier-Vidal, A. Laminin promotes attachment and neurite elongation of fetal hypothalamic neurons grown in serum-free medium. Neurosci. Letters 44: 83–89; 1984.
Farquhar, M. G. Fine structure and function in capillaries of the anterior pituitary gland. Angiology 12: 270–292; 1961.
Hogan, B. L. M.; Cooper, A. R.; Kurkinen, M. Incorporation into Reichert's membrane of laminin-like extracellular proteins synthesized by parietal endoderm cells of the mouse embryo. Developm. Biol. 80: 289–300; 1980.
Laurie, G. W.; Leblond, C. P.; Martin, G. R. Localization of type IV collagen, laminin, heparan sulfate proteoglycan and fibronectin to the basal lamina of basement membranes. J. Cell Biol. 95: 340–344; 1982.
Laurie, G. W.; Leblond, C. P.; Martin, G. R.; Silver, M. H. Intracellular localization of basement membrane precursors in the endodermal cells of the rat parietal yolk sac. III. Immunostaining for laminin and its precursors. J. Histochem. Cytochem. 30: 991–998; 1982.
Laurie, G. W.; Leblond, C. P.; Martin, G. R. Light microscopic immunolocalization of type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin in the basement membranes of a variety of rat organs. Amer. J. Anat. 167: 71–82; 1983.
Laurie, G. W.; Leblond, C. P. What is known of the production of basement membrane components. J. Histochem. Cytochem. 31: 159–163; 1983.
Liesi, P.; Dahl, D.; Vaheri, A. Laminin is produced by early rat astrocytes in primary culture. J. Cell Biol. 96: 920–924; 1983.
Madri, J. A.; Roll, F. J.; Furthmayr, H.; Foidart, J. M. Ultrastructural localization of fibronectin and laminin in the basement membranes of the murine kidney. J. Cell Biol. 86: 682–687; 1980.
Ohtsuki, I.; Manzi, R. M.; Palade, G. E.; Jamieson, J. D. Entry of macro-molecular tracers into cells fixed with low concentrations of aldehydes. Biol. Cell. 31: 119–126; 1978.
Olivier, L.; Vila-Porcile, E. Les lames basales des cordons épithéliaux du lobe antérieur de l'hypophyse chez le Rat. C. R. Soc. Biol. 166: 1413–1416; 1972.
Timpl, R.; Rohde, H.; Robey, P. G.; Rennard, S. I.; Foidart, J. M.; Martin, G. R. Laminin-A glycoprotein from basement membranes. J. Biol. Chem. 254: 9933–9937; 1979.
Tokuyasu, K. T. A technique for ultracryotomy of cell suspension and tissues. J. Cell biol. 57: 551–565; 1973.
Tougard, C.; Kerdelhué, B.; Tixier-Vidal, A.; Jutisz, M. Light and electron microscope localization of binding sites of antibodies against ovine luteinizing hormone and its two subunits in rat adenohypophysis using peroxidase labeled antibody technique. J. Cell Biol. 58: 503–521; 1973.
Tougard, C.; Picart, R.; Tixier-Vidal, A. Electron-microscopic cytochemical studies on the secretory process in rat prolactin cells in primary culture. Amer. J. Anat. 158: 471–490; 1980.
Author information
Authors and Affiliations
Additional information
This work was supported by grants from CNRS (Grant E.R. 89 and ATP “Pharmacologie des Récepteurs des Neuromediateurs”). Part of this work was performed at the EMBL (Heidelberg) during a short stay of C. Tougard (supported by an EMBO short term fellowship).
Rights and permissions
About this article
Cite this article
Tougard, C., Louvard, D., Picart, R. et al. Immunocytochemical localization of laminin in rat anterior pituitary cells in vivo and in vitro. In Vitro Cell Dev Biol 21, 57–61 (1985). https://doi.org/10.1007/BF02620915
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02620915