Summary
The Animal Cell Culture Collection established by the Advisory Committee and cooperating laboratories at the American Type Culture Collection has been described. The description includes procedures and criteria for the acceptance and certification of cells, guidelines for future studies, and policies for the selection of cells. The aim of this Collection is to fulfill the needs of individual investigators for characterized reference cells in broad areas of biological and medical research. The aid of interested investigators in anticipating and meeting these needs is earnestly solicited.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
American Type Culture Collection: Registry of animal cell lines certified by the Cell Culture Collection Committee. 1964, Supplements (1965, 1967) to First Edition. U.S. Public Health Service.
Cell Culture Collection Committee. 1964. Animal cell strains. Science146: 241–243.
Syverton Memorial Symposium on Analytic Cell Culture, 1962. R. E. Stevenson, ed. Nat. Cancer Inst. Monograph7: 252 pages.
Stulberg, C. S., Peterson, W. D., Jr. and Berman, L. 1962. Quantitative and qualitative preservation of cell-strain characteristics. Nat. Cancer Inst. Monograph7: 17–32.
Stulberg, C. S. 1963. Preservation and characterization of animal cell strains. InCulture Collections: Perspectives and Problems. Toronto: University of Toronto Press (p. 163–170).
Peterson, W. D., Jr. and Stulberg, C. S. 1964. Freeze preservation of cultured animal cells. Cryobiology1: 80–86.
Stulberg, C. S. and Peterson, W. D., Jr. 1966. Problems and experience in the characterization and preservation of tissue culture cells. Quart. Rev. Biol.41: 124–130.
Coriell, L. L., Greene, A. E. and Silver, R. D. 1964. Historical development of cell and tissue culture freezing. Cryobiology1: 72–79.
Greene, A. E., Athreya, B., Lehr, H. B. and Coriell, L. L. 1967. Viability of cell cultures following extended preservation in liquid nitrogen. Proc. Soc. Exp. Biol. and Med.124: 1302–1307.
Committee on Terminology. 1966. S. Fedoroff, Chairman. Proposed usage of animal tissue culture terms. In Vitro2: 155–159.
Brand, K. G. and Syverton, J. T. 1960. Immunology of cultivated mammalian cells. 1. Species specificity determined by hemagglutination. J. Nat. Cancer Inst.24: 1007–1019.
Coombs, R. R. A., Daniel, M. R., Gurner, B. W. and Kelus, A. 1961. Recognition of the species of origin of cells in culture by mixed agglutination. 1. Use of antisera to red cells. Immunology4: 55–66.
Stulberg, C. S., Simpson, W. F. and Berman, L. 1961. Species-related antigens of mammalian cell strains as determined by immunofluorescence. Proc. Soc. Exp. Biol. Med.108: 434–439.
Franks, D., Gurner, B. W., Coombs, R. R. A. and Stevenson, R. 1963. Results of tests for the species of origin of cell lines by means of the mixed agglutination reaction. Exp. Cell Res.28: 608–612.
Simpson, W. F. and Stulberg, C. S. 1963. Species identification of animal cell strains by immunofluorescence. Nature199: 616–617.
Greene A. E., Coriell L. L. and Charney, J. 1964. A rapid cytotoxic antibody test to determine species of cell cultures. J. Nat. Cancer Inst.32: 779–786.
Franks, D. and Gurner, B. W. 1965. Leucocyte alloantigens as markers on cultured human cells. Exp. Cell Res.37: 693–696.
Greene, A. E., Goldner, H. and Coriell, L. L. 1966. The species identification of poikilothermic tissue culture cells by the cytotoxic antibody test. Growth30: 305–313.
Rothfels, K. H., Axelrad, A. A., Siminovitch, L., McCulloch, E. A. and Parker, R. C. 1959. The origin of altered cell lines from mouse, monkey, and man, as indicated by chromosome and transplantation studies. InProceedings of the Third Canadian Cancer Research Conference, 1958, R. W. Begg, ed. New York: Academic Press, Inc. (p. 189–214).
Defendi, V., Billingham, R. E., Silvers, W. K. and Moorhead, P. F. 1960. Immunological and karyological criteria for identification of cell lines. J. Nat. Cancer Inst.25: 359–385.
Harris, M. 1964:Cell Culture and Somatic Variation. New York: Holt, Rinehart and Winston, pp. 547.
Merchant, D. J., Gangal, S. J. and Holmgren, N. B. 1966. Stability of cell antigens and their value as genetic markers. In Vitro2: 97–106.
Franks, D. 1966. Antigenic heterogeneity in cultures of mammalian cells. In Vitro2: 74–81.
Brand, K. G. and Chiu, S. Y. 1966. Antigenic analysis of cells and tissues; Differentiation between human cell culture strains. Nature212: 44–46.
Krooth, R. S. 1965. The future of mammalian cell genetics InNew Horizons in Human Genetics, J. German, ed. Birth Defects Original Article Series1: 21–56.
Hayflick, L. and Moorhead, P. F. 1961. The serial cultivation of human diploid cell strains. Exp. Cell Res.25: 585–621.
Gartler, S. M. 1967. Genetic markers as tracers in cell culture. InThe Decennial Review Conference on Cell Tissue and Organ Culture. Nat. Cancer Inst. Monograph26: 167–181.
Harris, H. 1966. Enzyme polymorphisms in man. Proc. Roy. Soc. London164: 298–310.
Conference on multiple molecular forms of enzymes. 1966. Ann. N. Y. Acad. Sci.151: 689 pages.
Yasin, R. and Goldenberg, G. J. 1966. Examination of isoenzymes of several dehydrogenases in pure cell lines. Nature211 1296–1297.
Ruddle, F. H., Shows, T. B. and Roderick, T. H. 1968. Autosomal control of an electrophoretic variant of glucose-6-phospate dehydrogenase in the mouse (Musmusculus). Genetics58: 599–606.
Peterson, W. D., Jr., Stulberg, C. S., Swanborg, N. K. and Robinson, A. R. 1968. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. and Med.128: 772–778.
Coriell, L. L. 1962. Detection and elimination of contaminating organisms. InAnalytic Cell Culture, R. E. Stevenson, ed. Nat. Cancer Inst. Monograph7: 33–53.
Barile, M. F., Malizia, W. F. and Briggs, D. B. 1962. Incidence and detection of pleuropneumonia-like organisms in cell culture by fluorescent antibody and cultural procedures. J. Bacteriology84: 130–136.
Coriell, L. L., McGarrity, G. J. and Horneff, J. 1967. Medical applications of dust free rooms. I. Elimination of airborne bacteria in a research laboratory. Amer. J. Public Health57: 1824–1836.
Coriell, L. L., Blakemore, W. S. and McGarrity, G. J. 1968. Medical applications of dust free rooms. II. Elimination of airborne bacteria from an operating theater. J. Am. Med. Assoc.203: 1038–1046.
Hayflick L. 1965. The limitedin vitro lifetime of human diploid cell strains. Exp. Cell. Res.37: 614–636.
Foley, G. E., Lazarus, H., Farber, S., Uzman, B. G., Boone, B. A. and McCarthy, R. E. 1965. Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer18: 522–529.
Moore, G. E., Ito, E., Ulrich, K. and Sandberg, A. A. 1966. Culture of human leukemia cells. Cancer19: 713–723.
Clarkson, B., Strife, A. and DeHarven, E. 1967. Continuous culture of seven new cell lines (SK-L 1 to 7) from patients with acute leukemia. Cancer20: 926–947.
Foley, G. E., Lazarus, H., Farber, S., Uzman, B. G. and Adams, R. A. 1968. Studies on human leukemic cells in vitro. InThe Proliferation and Spread of Neoplastic Cells. Univ. of Texas, M. D. Anderson Hospital and Tumor Inst. Symposium. Monograph21, p. 65–97.
Moore, G. E., Gerner, R. E. and Franklin, H. A. 1967. Culture of normal human leucocytes. J. Am. Med. Assoc.199: 519–524.
Epstein, M. S., Achong, B. G. and Barr, Y. M. 1964. Virus particles in cultured lymphoblasts from Burkitt's lymphoma. Lancet1: 702–703.
Henle, G., Henle, W. and Diehl, V. 1968. Relation of Burkitt's tumor-associated herpes-type virus to infectious mononucleosis. Proc. Nat. Acad. Sci. U.S.A.59: 94–101.
Franks, D. 1968. Antigens as markers on cultured mammalian cells. Biol. Rev.43: 17–50.
White, P. R. and Waymouth, C. 1954. Chemically defined synthetic nutrients for tissue cultures for pharmacological studies. Ann. N.Y. Acad. Sci.58:1023–1028.
Morgan, J. F., Campbell, M. E. and Morton, H. J. 1955. The nutrition of animal tissues cultivatedin vitro. 1. A survey of natural materials as supplements to synthetic medium 199. J. Nat. Cancer Inst.16: 557–567.
Sanford, K. K., Dupree, L. T. and Covalesky, A. B. 1963. Biotin, B12, and other vitamin requirements of a strain of mammalian cells grown in chemically defined medium. Exp. Cell. Res.31: 345–375.
German, J. L., Evans, V. J., Cortner, J. A. and Westfall, B. B. 1964. Characterization of three human cell lines by chromosomal complement and by certain biochemical parameters. Reversible alteration of isozyme patterns by different media. J. Nat. Cancer Inst.32: 681–707.
Evans, V. J., Bryant, J. C., Kerr, H. A. and Schilling, E. L. 1964. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res.36: 439–474.
Evans, V. J., Parker, G. A. and Dunn, T. B. 1964. Neoplastic transformations in C3H mouse embryonic tissuein vitro determined by intra-ocular growth. I. Cells from chemically defined medium with and without serum supplement. J. Nat. Cancer Inst.32: 89–121.
Eagle, H. 1965. Metabolic controls in cultured mammalian cells. Science,148: 42–51.
Eagle, H., Washington, C. and Friedman, S. M. 1966. The synthesis of homocystine, cystathionine, and cystine by cultured diploid and heteroploid human cells. Proc. Nat. Acad. Sci56: 156–163.
Fink, M. A., Karon, M., Rauscher, F. J., Malmgren, R. A. and Orr, H. C. 1965. Further observations on the immunofluorescence of cells in human leukemia. Cancer18: 1317–1321.
Epstein, M. A., Achong, B. G., Barr, Y. M., Zajac, B., Henle, G. and Henle, W. 1966. Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). J. Nat. Cancer Inst.37: 547–559.
Henle, G. and Henle, W. 1966. Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bacteriology91: 1248–1256.
Finegold, I., Fahey, J. L. and Granger, H. 1967. Synthesis of immunoglobulins by human cell lines in tissue culture. J. Immunol.,99: 839–848.
Tanjgaki, N., Yagi, Y., Moore, G. E. and Pressman, D. 1966. Immunoglobulin production in human leukemia cell lines. J. Immunol.,97: 634–646.
Kirsten, W. H., ed. 1966,Malignant Transformation by Viruses. New York: Springer-Verlag, p. 1–177.
Rapp, F. and Melnick, J. L. 1964. Application of tissue culture methods in the virus laboratory. InProgress in Medical Virology, J. L. Melnick, ed. Vol. 6. New York: Hafner Publishing Co. p. 268–317.
Yasumura, Y., Tashjian, Jr., A. H. and Sato, G. H. 1966. Establishment of four functional clonal strains of animal cells in culture. Science154: 1186–1189.
Cell Cultures for Virus Vaccine Production. November, 1967. A conference for the Division of Biologics Standards. D. J. Merchant, ed. Nat. Cancer Inst. Monograph,29: 608 pages.
Author information
Authors and Affiliations
Additional information
Supported by USPHS Grants CA 02947 (Child Research Center of Michigan); CA 04953 (Institute for Medical Research); CA 04975 (ATCC); and contract PH 43-63-13 (Naval Biological Laboratory); from the National Cancer Institute.
The Animal Cell Culture Collection was formed by the Cell Culture Collection Committee in 1960. This committee later became the Advisory Committee to the Animal Cell Culture Collection of the American Type Culture Collection (ATCC). Its present membership includes investigators from laboratories cooperating in the program and others as follows: C. S. Stulberg, (Chairman), L. L. Coriell, L. Hayflick, A. J. Kniazeff (for S. H. Madin), D. J. Merchant, H. R. Morgan, F. H. Ruddle, K. K. Sanford, R. E. Stevenson, and J. E. Shannon,Secretary ex officio.
Acknowledgments. The authors wish to thank members of the Committee as well as the following individuals for contributions and advice in the preparation of this article: F. H. Ruddle, W. D. Peterson, Jr., A. E. Greene, W. Nelson-Rees, M. Macy, F. Montes de Oca, W. W. Nichols, J. Charney, and W. F. Simpson.
Rights and permissions
About this article
Cite this article
Stulberg, C.S., Coriell, L.L., Kniazeff, A.J. et al. The animal cell culture collection. In Vitro 5, 1–16 (1970). https://doi.org/10.1007/BF02618370
Issue Date:
DOI: https://doi.org/10.1007/BF02618370