Abstract
Yersinia intermedia sp. nov. is defined biochemically and genetically.Y. intermedia strains belong to a single DNA relatedness group that is separable fromY. enterocolitica, Y. frederiksenii, Y. kristensenii, andY. pseudotuberculosis. Y. intermedia is positive in reactions for melibiose, raffinose, α-methyl-d-glucoside, rhamnose, and usually Simmons' citrate. It is metabolically more active at 22–28°C than at 35–37°C. These positive reactions serve to distinguishY. intermedia fromY. enterocolitica andY. kristensenii. Positive melibiose, raffinose, and α-methyl-d-glucoside reactions differentiateY. intermedia from the other new rhamnose-positive species,Y. frederiksenii. Y. intermedia is separated fromY. pseudotuberculosis by its positive reactions for sucrose, indole, cellobiose,i-inositol,d-sorbitol, α-methyl-d-glucoside, and ornithine decarboxylase.Yersinia biotype X2, an additional rhamnose-positive, sucrose-negative group, as yet not identified to a species, and X1, a sucrose- and ornithine decarboxylase-negativeYersinia biotype, do not belong toY. intermedia. Strain 3953 (=CIP 80-28=ATCC 29909=Bottone 48=Chester 48) is proposed as the type strain forY. intermedia.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literature Cited
Aldova, E., Lim, D. 1974.Yersinia enterocolitica in small rodents. Communication I: Pilot study in two wildlife areas. Zentralblatt für Bakteriologie, Parasitenkunde. Infektionskrankheiten und Hygiene, Abt. I. Orig., Reihe A226:491–496.
Alonso, J. M., Bejot, J., Bercovier, H., Mollaret, H. H. 1975. Sur un groupe de souches deYersinia enterocolitica fermentant le rhamnose. Médecine et Maladies Infectieuses10:490–492.
Alonso, J. M., Bercovier, H. 1975. Premers isolements en France deYersinia enterocolitica chez des micromammifères sauvages. Médecine et Maladies Infectieuses5:180–181.
Bercovier, H., Alonso, J. M., Bentaiba, Z. N., Brault, J., Mollaret, H. H. 1979. Contribution to the definition and the taxonomy ofYersinia enterocolitica. Contributions to Microbiology and Immunology5:12–22.
Bercovier, H., Brault, J., Barré, N., Treignier, M., Alonso, J. M., Mollaret, H. H. 1978. Biochemical, serological, and phage typing characteristics of 459Yersinia strains isolated from a terrestrial ecosystem. Current Microbiology1:353–357.
Bercovier, H., Brenner, D. J., Ursing, J., Steigerwalt, A. G., Fanning, G. R., Alonso, J. M., Carter, G. P., Mollaret, H. H. 1980. Characterization ofYersinia enterocolitica sensu stricto. Current Microbiology4:201–206.
Bercovier, H., Mollaret, H. H., Alonso, J. M., Brault, J., Fanning, G. R., Steigerwalt, A. G., Brenner, D. J. 1980. Intra-and interspecies relatedness ofYersinia pestis by deoxyribonucleic acid hybridization and its relationship toYersinia pseudotuberculosis. Current Microbiology4:225–230.
Bercovier, H., Ursing, J., Brenner, D. J., Steigerwalt, A. G., Fanning, G. R., Carter, G. P., Mollaret, H. H. 1980.Yersinia kristensenii: A new species of Enterobacteriaceae composed of sucrose-negative strains (formerly called atypicalYersinia enterocolitica orYersinia enterocolitica-like). Current Microbiology4:219–224.
Bottone, E. J. 1977.Yersinia enterocolitica: A panoramic view of a charismatic microorganism. CRC Critical Reviews in Microbiology5:211–241.
Bottone, E. J., Chester, B., Malowany, M., Allerhand, J. 1974. UnusualYersinia enterocolitica isolates not associated with mesentery lymphadenitis. Journal of Applied Microbiology27:858–861.
Botzler, R. G., Wetzler, T. F., Cowan, A. B. 1968.Yersinia enterocolitica andYersinia-like organisms isolated from frogs and snails. Bulletin of the Wildlife Disease Association.4:110–115.
Brenner, D. J., Fanning, C. R., Johnson, K. E., Citarella, R. V., Falkow, S. 1969. Polynucleotide sequence relationships among members of theEnterobacteriaceae. Journal of Bacteriology98:637–650.
Brenner, D. J., Fanning, G. R., Rake, A., Johnson, K. E. 1969. A batch procedure for thermal elution of DNA from hydroxyapatite. Analytical Biochemistry28:447–459.
Brenner, D. J., Steigerwalt, A. G., Falcao, D. P., Weaver, R. E., Fanning, G. R. 1976. Characterization ofYersinia enterocolitica andYersinia pseudotuberculosis by deoxyribonucleic acid hybridization and by biochemical reactions. International Journal of Systematic Bacteriology26:180–194.
Brenner, D. J., Steigerwalt, A. G., Miklos, G. V., Fanning, G. R. 1973. Deoxyribonucleic acid relatedness among erwiniae and otherEnterobacteriaceae: The soft-rot organisms (genusPectobacterium Waldee). International Journal of Systematic Bacteriology23:205–216.
Brenner, D. J., Ursing, J., Bercovier, H., Steigerwalt, A. G., Fanning, G. R., Alonso, J. M., Mollaret, H. H. 1980. Deoxyribonucleic acid relatedness inYersinia enterocolitica andYersinia enterocolitica-like organisms. Current Microbiology4:195–200.
Dabernat, H. J., Bauriaud, R., Lemozy, J., Lefevre, J. C., Lareng, M. B. 1978.Yersinia enterocolitica fermentant le rhamhose. A propos de 15 souches isolées chez des enfants. Médecine et Maladies Infectieuses8:158–162.
Hanna, M. O., Zink, D. L., Carpenter, Z. L., Vanderzant, C. 1976.Yersinia enterocolitica-like organisms from vacuum-packaged beef and lamb. Journal of Food Science41:1254–1256.
Harvey, S., Greenwood, J. R., Pickett, M. J., Mah, R. A. 1976. Recovery ofYersinia enterocolitica from streams and lakes of California. Applied and Environmental Microbiology32:352–354.
Highsmith, A. K., Feeley, J. C., Skaliy, P., Wells, J. G., Wood, B. T. 1977. Isolation ofYersinia enterocolitica from well water and growth in distilled water. Applied and Environmental Microbiology34:745–750.
Kapperud, G. 1977.Yersinia enterocolitica andYersinia-like microbes isolated from mammals and water in Norway and Denmark. Acta Pathologica et Microbiologica Scandinavica85B:129–135.
Lassen, J. 1972.Yersinia enterocolitica in drinking-water. Scandinavian Journal of Infectious Diseases4:125–127.
Mandel, M., Marmur, J. 1968. Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods in Enzymology12B:195–206.
Mandel, M., Schildkraut, C. L., Marmur, J. 1968. Use of CsCI density gradient analysis for determining the guanine plus cytosine content of DNA. Methods in Enzymology12B:184–195
Matthew, M., Cornelis, G., Wauters, G. 1977. Correlation of serological and biochemical groupings ofYersinia enterocolitica with the beta-lactamases of the strains. Journal of General Microbiology102:55–59.
Owen, R. J., Hill, L. R., Lapage, S. P. 1969. Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers7:503–516.
Schiemann, D. A. 1978. Isolation ofYersinia enterocolitica from surface and well waters in Ontario. Canadian Journal of Microbiology102:55–59.
Steigerwalt, A. G., Fanning, G. R., Fife-Asbury, M. A., Brenner, D. J. 1976. DNA relatedness among species ofEnterobacter andSerratia. Canadian Journal of Microbiology22:121–137.
Ursing, J., Brenner, D. J., Bercovier, H., Fanning, G. R., Steigerwalt, A. G., Brault, J., Mollaret, H. H. 1980.Yersinia frederiksenii: A new species of Enterobacteriaceae composed of rhamnose-positive strains (formerly called atypicalYersinia enterocolitica orYersinia enterocolitica-like). Current Microbiology4:213–218.
Wauters, G., Le Minor, L., Chalon, A. M. 1971. Antigènes somatiques et flagellaires deYersinia enterocolitica. Annales de l'Institut Pasteur120:631–642.
Wetzler, T. F., Hubbert, W. T. 1968.Yersinia enterocolitica in North America. International Symposium on Pseudotuberculosis9:343–356.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brenner, D.J., Bercovier, H., Ursing, J. et al. Yersinia intermedia: A new species of enterobacteriaceae composed of rhamnose-positive, melibiose-positive, raffinose-positive strains (formerly calledYersinia enterocolitica orYersinia enterocolitica-like). Current Microbiology 4, 207–212 (1980). https://doi.org/10.1007/BF02605858
Issue Date:
DOI: https://doi.org/10.1007/BF02605858