Abstract
Single-strand conformational polymorphism (SSCP) gel electrophoresis and DNA sequencing were used to characterize the second exon of the horseDRB homologue as well as to identify eight newDRB alleles. The SSCP gels presented a complex pattern, with phenotypes exhibiting between 4 and 13 bands. TheDRB SSCP patterns were studied for two families (6 to 13 bands per pattern). For both families, the patterns showed simple Mendelian inheritance. The polymerase chain reaction products from two individuals possessing homozygous major histocompatibility complex (MHC) alleles by descent were cloned and retested on SSCP gels. All bands derived from the genomic DNA amplification could be accounted for with bands derived from the cloned DNA amplification products. The results were consistent with threeDRB loci, though this number may be variable within the domestic horse population. Gene sequences were variable among the different products, and we were unable to assign locus designations for particular sequences. Amplification of cDNA library material derived from one of the individuals who isMHC homozygous by descent showed an SSCP profile suggesting that all threeDRB loci are transcribed into mRNA.
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The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers L76972-L76978 and L77079
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Fraser, D.G., Bailey, E. Demonstration of threeDRB locl in a domestic horse family. Immunogenetics 44, 441–445 (1996). https://doi.org/10.1007/BF02602805
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DOI: https://doi.org/10.1007/BF02602805