Summary
Electron microscopic examination of bone biopsies obtained from two patients suffering from pycnodysostosis revealed that osteoclasts contained (sometimes large) cytoplasmic vacuoles filled with bone collagen fibrils. These vacuoles stained positive for acid phosphatase activity, thereby suggesting that bone matrix had been phagocytosed and subsequently exposed to hydrolytic enzymes of the lysosomal apparatus. Collagen-containing vacuoles were not observed in osteoclasts of individuals not suffering from this disease.
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References
Bonucci E (1974) The organic-inorganic relationships in bone matrix undergoing osteoclastic resorption. Calcif Tissue Res 16:13–36
Bonucci E (1981) New knowledge on the origin, function and fate of osteoclasts. Clin Orthop Rel Res 158: 252–269
Hancox NM, Boothroyd B (1961) Motion picture and electron microscope studies on the embryonic avian osteoclast. J Bioph Biochem Cyt 11:651–661
Vaes G (1980) Collagenase, lysosomes and osteoclastic bone resorption. In: Woolley DE, Evanson JM (eds) Collagenase in normal and pathological connective tissues. John Wiley, Chichester, New York, Brisbane, Toronto, p 185
Heersche JNM (1978) Mechanisms of osteoclastic bone resorption: a new hypothesis. Calcif Tissue Res 26:81–84
Sakamoto S, Sakamoto M (1982) Biochemical and immunohistochemical studies on collagenase in resorbing bone in tissue culture. A novel hypothesis for the mechanism of bone resorption. J Periodont Res 17:523–526
Maroteaux P, Lamy M (1962) Deux observations d'une affection osseuse condensante: la pycnodysostose. Arch Fr Paediatr 19:267–274
Sedano HD, Gordin RJ, Anderson VE (1968) Pycnodysostosis. Am J Dis Child 116:70–77
Elmore SM (1967) Pycnodysostosis: a review. J Bone Joint Surg 49A:153–162
Aronson DC, Heymans HSA, Bijlmer RPGM (1984) Cor pulmonale and acute liver necrosis, due to upper airway obstruction as part of pycnodysostosis. Eur J Pediatr 141:251–253
Bressot C, Meunier PJ, Bard J, Vignon G (1980) La pycnodysostose. Analyse histomorphometrique et dynamique de l'os dans une nouvelle observation. Rev Rhumatisme 47:425–430
Meredith SC, Simon MA, Laros GS, Jackson MA (1978) Pycnodysostosis. A clinical, pathological, and ultramicroscopic study of a case. J Bone Joint Surg 60:1122–1127
Stanescu R, Stanescu V, Maroteaux P (1975) Anomalies ultrastructurales des chondrocytes de la pycnodysostose. Nouv Presse Med 4:2647–2650
Stanescu V, Stanescu R, Maroteaux P (1973) Etude histochimique et microchimique du cartilage de croissance tibial dans le nanisme diastrophique et la pycnodysostose. Ann Histochimie 18:179–186
Barka T, Anderson PJ (1962) Histochemical method, for acid phosphatase using hexazonium pararosanilin as coupler. J Histochem Cytochem 10:741–753
De Bruijn WC, Schellens JPM, Van Buitenen JMH, Van der Meulen J (1980) X-Ray microanalysis of colloidal-gold-labelled lysosomes in rat liver sinusoidal cells after incubation for acid phosphatase activity. Histochem 66:137–148
Göthlin G, Ericsson JLE (1976) The osteoclast. Review of ultrastructure, origin, and structure-function relationship. Clin Orthop Rel Res 120:201–231
Holtrop ME, King GJ (1977) The ultrastructure of the osteoclast and its functional implications. Clin Orthop Rel Res 123:177–196
Lucht U (1971) Acid phosphatase of osteoclasts demonstrated by electron microscopic histochemistry. Histochem 28:103–117
Hancox NM (1972) The osteoclast. In: Bourne GH (ed) The biochemistry and physiology of bone, vol. 1 Academic Press, New York, San Francisco, London, p 452
Lucht U (1972) Cytoplasmic vacuoles and bodies of the osteoclast. Z Zellforsch 135:229–244
Thyberg J (1975) Electron microscopic studies on the uptake of exogenous marker particles by different cell types in the guinea pig metaphysis. Cell Tissue Res 156:301–315
Melcher AH, Chan J (1981) Phagocytosis and digestion of collagen by gingival fibroblasts in vivo: a study of serial sections. J Ultrastruct Res 77:1–36
Fullmer HM, Lazarus G (1967) Collagenase in human, goat and rat bone. Isr J Med Sci 3:758–761
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Everts, V., Aronson, D.C. & Beertsen, W. Phagocytosis of bone collagen by osteoclasts in two cases of pycnodysostosis. Calcif Tissue Int 37, 25–31 (1985). https://doi.org/10.1007/BF02557674
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DOI: https://doi.org/10.1007/BF02557674