Abstract
The effect of immobilization on human bone was studied through a longitudinal, as well as cross-sectional, quantitative and dynamic histological analysis of 34 decalcified and undecalcified iliac crest biopsies. They were obtained at various times after the onset of immobilization in 28 patients of which 22 were suffering from post-traumatic spinal cord lesions. Trabecular bone volume, osteoid volume, trabecular osteoclastic resorption surfaces, size of the periosteocytic lacunae, thickness of iliac cortices and volume of the cell population of the marrow were measured. The histodynamic study was made by double tetracycline labeling in 19 patients. The histological data were compared with biological data from another group of 68 immobilized patients including 22 of the patients undergoing biopsy. Calcemia, phosphoremia, alkaline phosphatase, calciura, phosphaturia and hydroxyprolinuria were measured. The decrease of the trabecular bone volume averaged 33% over 25 weeks and then stabilized. Immobilization also caused an early increase in the trabecular osteoclastic resorption surfaces and later in the size of periosteocytic lacunae, an early depression of osteoblastic bone formation and a thinning of the cortices. Calciuria was high, as was hydroxyprolinuria which correlates with resorption surfaces. The histological and biochemical changes suggest an histodynamic hypothesis according to which the global lifespan of the BMU (Basic Multicellular Unit from Frost) would be increased. These changes reflect atransient, leading to a newsteady state: rarefied bone with a low rate of subsequent turn-over. They emphasize the importance of mechanical factors in the development of bone cells.
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Albright, F., Burnett, C. H., Gope, O., Parsons, W.: Acute atrophy of bone (osteoporosis) simulating hyperparathyroidism. J. clin. Endocr.1, 711 (1941).
Arnstein, A. R., McCann, D. S., Blumenthal, F. S., Prunty, J.: Serum immunoreactive parathyroid hormone in patients with paralysis due to spinal cord trauma. Clinical aspects of metabolic bone disease. Frame, B., Parfitt, A. M., Duncan, H., (eds.). International Congress Series No. 270, p. 253–256. Excerpta medica, Amsterdam 1973.
Bassett, C. A. L.: Biologic significance of piezo-electricity. Calcif. Tis. Res.1, 252–272 (1968).
Briggs, A. P.: A modification of the Bell-Doisy phosphate method. J. biol. Chem.53, 13–18 (1922)
Bruce, D. S., Wiebers, J. E.: Mineral dynamics during hibernation and chronic immobility: review. a Aerospace Med.40, 855–861 (1969)
Burckhart, J. M., Jowsey, J.: Parathyroid and thyroid hormones in the development of immobilisation osteoporosis. Endocrinology81, 1053–1062 (1967)
Cates, R. J., Sheets, D. D., Johnston, C. C.: Bone atrophy in the paralyzed limb and attempted arrest. Clin. Res.18, 622–625 (1970)
Chantraine, A.: Clinical investigation of bone metabolism in spinal cord lesions. Paraplegia8, 253–259 (1971)
Courpron, P., Meunier, P., Edouard C., Bernard, J., Bringuier, J., Vignon, G.: Données histologiques quantitatives sur le vieillissement osseux humain. Rev. Rhum.7–9, 469–483 (1973)
Epker, B. N., Frost, H. M.: Correlation of bone resorption and formation behavior of loaded bone. J. Dent. Res.44, 33–41 (1965)
Frost, H. M.: Bone remodeling and metabolic bone disease. Orthopaedic lecture series, vol. III. Springfield: Charles C. Thomas 1972.
Furman, R., Nicholas, J. J., Jivoff, L.: Elevation of the serum alkaline phosphatase coincident with ectopic bone formation in paraplegic patients. J. Bone Jt Surg. A52, 1131–1137 (1970)
Hardt, A. B.: Early metabolic responses of bone to immobilization. J. Bone Jt Surg. A54, 119–124 (1972)
Heaney, R. P.: Radiocalcium metabolism in disuse osteoporosis in man. Amer. J. Med.2 188–200 (1972)
Kazarian, L. E., Gierke, H. E. von: Bone loss as a result of immobilization and chelation, preliminary results in Macaca mulatta. Clin. Orthop.65, 67–76 (1969)
Klein, B., Read, P. A., Babson, A. L.: Rapid method for the quantitative determination of serum alkaline phosphatase. Clin. Chem.6, 269–275 (1960)
Klein, L., Kanefield, D. G., Heiple, K. G.: Effect of disuse osteoporosis on bone composition: the fate on bone matrix. Calcif. Tis. Res.2, 20–29 (1968)
Landry, M., Fleisch, H.: The influence of immobilization of bone formation as evaluated by osseous incorporation of tetracycline. J. Bone Jt Surg. B46, 764–771 (1964)
Lutwak, L., Whedon, G. D., Lachance, P. A., Reid, J. M., Lipscomp, H. S.: Mineral electrolyte and nitrogen balance studies of the Gemini-VII fourteen day orbital space flight. J. clin. Endocr.29, 1140–1156 (1969)
Mack, P. B., Lachance, R. A.: Effects of recumbency and space flight on bone density. Amer. J. clin. Nutr.20, 1194–1205 (1967)
Mattson, S.: The reversibility of disuse osteoporosis. Experimental studies in the adult rat. Acta orthop. scand., suppl. No.144 (1972)
Meunier, P., Bernard, J. Courpron, P., Vignon, G.: Use of an image analysing computer for the study of osteocytic behavior in bone diseases. Proceedings of 9th European Symposium on Calcified Tissues. Baden (Czitober, H., Eschberger, J., ed.), p. 203–208. Vienna: Facta Publication, 1973c
Meunier, P., Bianchi, G. G.-S., Edouard, C., Bernard, J., Courpron, P., Vignon, G.: Bony manifestations of thyrotoxicosis. Orthop. Clin. N. Amer.3, 745–774 (1972)
Meunier, P., Courpron, P., Edouard, C., Bernard, J., Bringuier, J., Vignon, G.: Physiological senile involution and pathological rarefaction of bone. Quantitative and comparative histological data. Clin. Endocr.2, 239–256 (1973b)
Meunier, P., Vignon, G., Bernard, J., Edouard, C., Courpron, P.: Quantitative bone histology as applied to the diagnosis of hyperparathyroid states. Clinical aspects of metabolic bone disease. Frame, B., Parfitt, A. M., Duncan, H., (eds.). International Congress Series No. 270, p. 215–223. Excerpta medica, Amsterdam 1973a
Prockop, D. J., Voenfriend, S.: A specific method for the analysis of hydroxyproline. J. clin. Invest.43, 453–460 (1964)
Semb, H.: Experimental disuse osteoporosis. Acta Soc. Med. upsalien.71, 83–107 (1966)
Sevastik, J. A., Mattson, S.: Osteoporosis and parathyroid glands. Studies on the volume and the activity of the parathyroid glands in disuse bone atrophy in adult rats. Israel J. med. Sci.7, 346–349 (1971)
Stellmann, C., Beranger, G.: Utilisation de régression curviligne multiple pour la recherche d’interaction entre plusieurs facteurs quantitatifs. Rev. Statistiques Appliquées (in press)
Tronchet, J.: La détermination complexométriquodu calcium sérique. Proposition d’une technique de dosage direct. Ann. Biol. Clin.16, 459–470 (1958)
Vignon, G., Meunier, P., Pansu, D., Bernard, J.: Enquête clinique et anatomique sur l’étiopathogénie de l’ostéoporose sénile. Rev. Rhum.37, 615–627 (1970)
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Minaire, P., Meunier, P., Edouard, C. et al. Quantitative histological data on disuse osteoporosis. Calc. Tis Res. 17, 57–73 (1974). https://doi.org/10.1007/BF02547214
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DOI: https://doi.org/10.1007/BF02547214