Abstract
Fatty acid composition of seed oil is determined in less than one hr using a quantitative one-vial technique. The method of analysis requires alcoholic solutions of sodium methoxide mild enough for epoxy and polyunsaturated oils. Separation and characterization of component fatty acids were accomplished by high resolution gas chromatography-mass spectrometry.
Using this method,Vernonia galamensis seed oil is shown to contain 79–80% vernolic (cis-12,13-epoxy-cis-9-octadecenoic) acid.Amaranthus cruentus, a West African vegetable crop, is shown to contain 17.3% palmitic acid, 3.2% stearic acid, 22.7% oleic acid, 54.7% linoleic acid and 2.1% linolenic acid.
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References
Official Methods of Analysis of the Association of Official Agricultural Chemists, Tenth Edition, AOAC Washington D.C., 1965, Method 26036.
Geeraert, E., and P. Sandra,J. Am. Oil Chem. Soc. 64:100 (1987).
Official Methods of Analysis of the Association of Official Agricultural Chemists, Tenth Edition, AOAC Washington, D.C., 1965, Method 26052.
Carlson, K.D., W.J. Schneider, S.P. Chang and L.H. Princen inNew Sources of Fats and Oils, edited by E.H. Pryde, L.H. Princen and K.D. Mukherjee, AOCS, Champaign, IL, 1981, pp. 297–318.
Perdue, P.E. Jr., K.D. Carlson and M.G. Gilbert,Econ. Bot. 40:54 (1986).
Princen, L.H.,J. Am. Oil Chem. Soc. 56:845 (1979).
Becker, R., E.L. Wheeler, K. Lorenz, A.E. Stafford, O.K. Grosjean, A.A. Betschart and R.M. Saunders,J. Food Sci. 46:1175 (1981).
Aziz, P., S.A. Khan and A.W. Sabir,Pakistan J. Sci. Ind. Res. 27:215 (1984).
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Ayorinde, F.O., Clifton, J., Afolabi, O.A. et al. Rapid transesterification and mass spectrometric approach to seed oil analysis. J Am Oil Chem Soc 65, 942–947 (1988). https://doi.org/10.1007/BF02544516
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DOI: https://doi.org/10.1007/BF02544516