Abstract
A new microbial isolate,Flavobacterium sp. strain DS5, converts linoleic acid into 10-hydroxy-12(Z)-octadecenoic acid (10-HOA) with 55% yield. The product was characterized by gas chromatography (GC), GC/mass spectrometry, nuclear magnetic resonance and Fourier transform infrared spectroscopy. The specific optical rotation of 10-HOA is [α] 24D =−5.58 (methanol). The optimum time, pH and temperature for the production of 10-HOA were 36h, 7.5 and 20–35°C, respectively. The enzyme(s) that converts linoleic acid to 10-HOA is soluble and located intracellulary in strain DS5. Two minor products, 10-methoxy-12-octade-cenoic acid and 10-keto-12-octadecenoic acid, were also identified. 10-HOA was further metabolized by strain DS5. Among the unsaturated fatty acids studied, the order of reactivity for the DS5 enzyme(s) is oleic>palmitoleic> linoleic>linolenic>γ-linolenic>myristoleic acid.
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Hou, C.T. Conversion of linoleic acid to 10-hydroxy-12(Z)-octadecenoic acid byFlavobacterium sp. (NRRL B-14859). J Am Oil Chem Soc 71, 975–978 (1994). https://doi.org/10.1007/BF02542264
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DOI: https://doi.org/10.1007/BF02542264