Abstract
Chlorophyll derivatives have been isolated from a degummed soybean oil by cellulose column chromatography and resolved by reversed phase HPLC. The HPLC separation was performed on a Zorbax ODS column using acetone-methanol (75:25) as the mobile phase. Seven major components were detected by visible (650 nm) light absorption. Pheophytin A is the predominant component of the mixture (40–45% of the total). Pheophytin A’, pyropheophytin A, and three unidentified pigments having spectral features resembling pheophytin A comprise the other major pigments. No evidence was found for the presence of chlorophylls A and B in this oil.
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Rawls, M.R., and P.J. van Santen, JAOCS 47:121 (1970).
Clements, A.H., R.H. van den Engh, D.J. Frost and K. Hoogenhout, JAOCS 50:325 (1973).
Carlsson, D.J., T. Suprunchuk and D.M. Wiles, JAOCS 53:656 (1976).
Sattar, A., and J.M. Deman, JAOCS 53:473 (1976).
Chan, H.W.-S., JAOCS 54:100 (1977).
Terao, J., and S. Matsushita, JAOCS 54:234 (1977).
Frankel, E.N., W.E. Neff and T.R. Bessler, Lipids 14:961 (1979).
Terao, J., and S. Matsushita, J. Food Processing and Preservation 3:329 (1980).
Terao, J.; R. Yamauchi, H. Murakami and S. Matsushita, J. Food Processing and Preservation 4:79 (1980).
Holden, M., in “Chemistry and Biochemistry of Plant Pigments,” 2nd edition, edited by T.W. Goodwin, Vol. 2, Academic Press, New York, 1976, pp. 1–37.
Vernon, L.P., Anal. Chem. 32:1144 (1960).
White, R.C.; I.D. Jones and E. Gibbs, J. Food Sci. 28:431 (1963).
White, R.C.; I.D. Jones, E. Gibbs and L.S. Butler, J. Agr. Food Chem. 20:773 (1972).
AOCS Official and Tentative Methods, American Oil Chemists’ Society, Champaign, IL, 1973, Method Cc 13d-55.
Pritchett, W.C.; W.G. Taylor and D.M. Carroll, JAOCS 24:225 (1947).
O’Connor, R.T.; E.T. Field, M.E. Jefferson and F.G. Dollear, JAOCS 26:710 (1949).
Mackinney, G., J. Biol. Chem. 132:91 (1940).
Wilson, J.R.; M-D. Nutting and G.F. Bailey, Anal. Chem. 34:1331 (1962).
Wasielewski, M.R., and W.A. Svec, J. Org. Chem. 45:1969 (1980).
Pennington, F.C.; H.H. Strain, W.A. Svec and J.J. Katz, J. Amer. Chem. Soc. 86:1418 (1964).
Strain, H.H., and W.A. Svec, in “The Chlorophylls,” L.P. Vernon and G.R. Seely, eds., Academic Press, New York, NY, 1966, p. 21.
Strain, H.H.; M.R. Thomas and J.J. Katz, Biochim. Biophys. Acta 75:306 (1963).
Sievers, G., and P.H. Hynninen, J. Chrom. 134:359 (1977).
Shiraki, M.; M. Yoshiura and K. Iriyama, Chem. Lett. 103 (1978).
Yoshiura, M.; K. Iriyama and M. Shiraki, Chem. Lett. 281 (1978).
De Jong, D.W., and W.G. Woodlief, J. Agric. Food Chem. 26:1281 (1978).
Rebeiz, C.A.; M.B. Bazzaz and F. Belanger, Chrom. Review 4:8 (1978).
Liebezeit, G., J. High Resolution Chrom. and Chrom. Comm. 3:531 (1980).
Schwartz, S.J.; S.L. Woo and J.H. von Elbe, J. Agric. Food Chem. 29:533 (1981).
Braumann, T., Biochim. Biophys. Acta 637:8 (1981).
Norris, F.A., and K.F. Mattil, in “Bailey’s Industrial Oil and Fat Products,” 3rd Ed., edited by D. Swern, John Wiley and Sons, New York, NY, 1964.
Wilson, J.R., and M.-D. Nutting, Anal. Chem. 35:144 (1963).
Niewiadomski, H.; I. Bratkowska and E. Mossakowska, JAOCS 42:731 (1965).
Aronoff, S., in “Adv. in Food Research,” Vol. IV, edited by E.M. Mrak and G.F. Stewart, Acad. Press, New York, NY, 1953, pp. 133–184.
Endo, Y.; R. Usuki and T. Kaneda, JAOCS 61:781 (1984).
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Fraser, M.S., Frankl, G. Detection of chlorophyll derivatives in soybean oil by HPLC. J Am Oil Chem Soc 62, 113–121 (1985). https://doi.org/10.1007/BF02541506
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DOI: https://doi.org/10.1007/BF02541506