Abstract
• Background: The possible existence and distribution patterns of α/β- and γ/δ-TCR+ cells, which are important constituents of immune surveillance and act via the CD3+ cell complex have not yet been elucidated in the healthy and inflamed conjunctiva. • Materials and methods: Paraffin-embedded conjunctival specimens included 18 from 18 patients with ocular cicatricial pemphigoid (OCP), 20 from 20 healthy controls, 6 from 6 patients with lye burns, and 6 from 2 patients with Stevens-Johnson syndrome; all were worked up by histology and immunohistochemistry. • Results: α/β-TCR+ cells were visualized in the conjunctival epithelium and stroma of healthy persons, OCP, lye burns and Stevens-Johnson syndrome. α/β-TCR+ cells and a small number of γ/δ-TCR+ cells were observed in the corneal epithelium and stroma of patients who have failing corneal grafts. After ileal mucosa transplantation to the epibulbar conjunctiva, membrane staining changes to nuclear and cytoplasmic staining. Treatment with systemic cytotoxic drugs abolishes all α/β-TCR+ and γ/δ-TCR+ cells. • Conclusions: α/β-TCRu+ cells can be found in the non-infected epithelium and stroma of the healthy and inflamed (OCP, lye burns, and Stevens-Johnson syndrome) conjunctiva, as well as in the corneal epithelium and stroma of failing corneal grafts, whereas γ/δ-TCR+ cells are absent. A small number of γ/δ-TCR+ cells are present in the corneal stroma and adjacent conjunctival epithelium of patients with chronic corneal graft rejection or after transplantation of gut tissue. Further investigations may establish the role, if any, of these T-cell subsets in immune surveillance of the non-infected outer eye and in corneal graft rejection.
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Bialasiewicz, A.A., Schaudig, U., Ma, JX. et al. α/β- andγ/δ-T cell-receptor-positive lymphocytes in healthy and inflamed human conjunctiva. Graefe's Arch Clin Exp Ophthalmol 234, 467–471 (1996). https://doi.org/10.1007/BF02539415
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DOI: https://doi.org/10.1007/BF02539415