Abstract
A very sensitive high performance liquid chromatographic (HPLC) method was developed for the separation of α-tocopherol (α-T) and its five oxidation products: α-tocopheryl quinone (TQ), dimer (D), dihydroxy dimer (DHD), trimer (T) and 9-methoxy-α-tocopherone commonly called α-tocopheroxide (TO). The separation was achieved on a normal-phase silica-based column (Ultrasphere-Si), using a mobile phase of hexane/chloroform/isopropanol (95∶4.5∶0.5, v/v/v) at a flow rate of 0.4 ml/min, and the eluants were monitored simultaneously at their maximum absorptions using a variable-wavelength UV detector. The minmum detection limit is 0.01 μg for α-T, TQ and TO, 0.05 μg for DHD and D, and 0.1 μg for T/injection. This normal-phase method has the combined advantages of being very sensitive, fast and capable of separating all six compounds at the same time.
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Abbreviations
- α-T:
-
α-tocopherol
- AUFS:
-
absorption unit full scale
- D:
-
dimer
- DHD:
-
dihydroxy dimer
- HPLC:
-
high performance liquid chromatography
- T:
-
trimer
- TO:
-
α-tocopheroxide
- TOP:
-
α-T oxidation products
- TQ:
-
α-tocopheryl quinone
References
Bieri, J.G., and Tolliver, T.J. (1981)Lipids 16, 777–779.
Csallany, A.S. (1971)Intl. J. Vit. Nutr. Res. 41, 376–384.
Csallany, A.S., Chiu, M., and Draper, H.H. (1970)Lipids 5, 63–70.
Csallany, A.S., and Draper, H.H. (1963)Arch. Biochem. Biophys. 100, 335–337.
Csallany, A.S., and Draper, H.H. (1963)J. Biol. Chem. 238, 2912–2915.
Strauch, B.S., Fales, H.M., Pittman, R.C., and Avigan, J. (1969)J. Nutr. 97, 194–202.
Draper, H.H., and Csallany, A.S. (1970)The Fat Soluble Vitamins (Deluca, H.F., and Suttie, J.W., eds.) pp. 347–353, University of Wisconsin Press, Madison, WI.
Buckley, J., and Connolly, J.F. (1980)J. Food Protection 43, 265–267.
Boguth, W., and Nienamm, H. (1971)Biochim. Biophys. Acta 248, 121–130.
Meijboom, P.W., and Jongentter, G.A. (1979)J. Am. Chem. Soc. 56, 33–35.
Osterlof, G., and Nyheim, P. (1980)J. Chromatogr. 183, 487–491.
Cillard, J., Cillard, P., and Cormier, M. (1980)J. Am. Oil Chem. Soc. 57, 252–255.
Chappel, J.E., Francis, T., and Clandinin, M.T. (1986)Nutr. Res. 6, 849–852.
Claude, F.B., George, P.R., and Cronenberger, L.A. (1984)J. Assoc. Off. Anal. Chem. 67, 627–632.
Zaspel, B.M., and Csallany, A.S. (1983)Anal. Chem. 130, 146–150.
Badcock, N.R., O'Reilly, D.A., and Pinnock, C.B. (1986)J. Chromatogr. 382, 290–296.
Bieri, J.G., Tolliver, T.J., and Catignani, G.L. (1979)Am. J. Clin. Nutr. 32, 2143–2149.
Cartignani, G.L., and Bieri, J.G. (1981)Clin. Chem 29, 708–712.
Stancher, B., and Zonta, F. (1983)J. Chromatogr. 256, 93–100.
Widicus, W.A., and Kirk, J.R. (1981)J. Food Sci. 46, 813–816.
Widicus, W.A., and Kirk, J.R. (1979)J. Assoc. Off. Anal. Chem. 82, 837–841.
Cillard, J., Gobaille, J., and Cillard, P. (1985)J. Chromatogr. 347, 434–437.
Eggitt, P.W.R., and Norris, F.W. (1956)J. Sci. Food Agri. 7, 493–511.
Boyer, P.D. (1951)J. Am. Chem. Soc. 73, 733–735.
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Ha, Y.L., Scallany, A.S. Separation of α-tocopherol and its oxidation products by high performance liquid chromatography. Lipids 23, 359–361 (1988). https://doi.org/10.1007/BF02537349
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DOI: https://doi.org/10.1007/BF02537349