Abstract
To evaluate the effects of strain, gender and fasting in the regulation of lipoprotein lipase (LPL) and hepatic lipase (HL) activities were measured in tissues of male and female Wistar and Sprague-Dawley rats after feeding or a 24-h starvation period. It is noteworthy that an effect of gender on LPL activity was observed in Wistar, but not in Sprague-Dawley rats, not in the basal (fed) activity in several tissues, such as white and brown adipose tissues, heart, and brain, but also in response to fasting which affected LPL activity in brown adipose tissue, heat and lung of female but not of male Wistar rats. By contrast, HL activity in liver, plasma and adrenals of Sprague-Dawley rats was higher in females than in males. No effect of gender on HL activity was observed in Wistar rats. Our results indicate that differences exist between Wistar and Sprague-Dawley rats in the regulation of both LPL and HL. Some of the contradictory results found in the literature may be explained by the differences between rat strains and gender, as well as differences in the nutritional status of the animals.
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Abbreviations
- BAT:
-
brown adipose tissue
- HDL:
-
high density lipoproteins
- Hepes:
-
N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
- HL:
-
hepatic lipase
- LPL:
-
lipoprotein lipase
- Pipes:
-
piperazine-N,N′-bis(2-ethanesulfonic acid)
- Tris:
-
tris(hydroxymethyl)aminomethane
- WAT:
-
white adipose tissue
References
Nilsson-Ehle, P., Garfinkel, A.S., and Schotz, M.C. (1980)Ann. Rev. Biochem. 49, 667–693.
Cryer, A. (1981)Int. J. Biochem. 13, 525–541.
Ramírez, I., Llobera, M., and Herrera, E. (1983)Metabolism 32, 333–341.
Vilaró, S., Llobera, M., Bengtsson-Olivecrona, G., and Olivecrona, T. (1988)Biochem. J. 249, 549–556.
Burgaya, F., Peinado, J., Vilaró, S., Llobera, M., and Ramírez, I. (1989)Biochem. J. 259, 159–166.
Camps, L., Reina, M., Llobera, M., Vilaró, S., and Olivecrona, T. (1990)Am. J. Physiol. 258, C673-C681.
Landin, B., Nilsson, A., Twu, J.S., and Schotz, M.C. (1984)J. Lipid Res. 25, 559–563.
Simard, G., and Perret, B. (1990)Ann. Biol. Clin. 48, 61–76.
Vilaró, S., Ramírez, I., Bengtsson-Olivecrona, G., Olivecrona, T., and Llobera, M. (1988)Biochim. Biophys. Acta 959, 106–117.
Laposata, E.A., Laboda, H.M., and Strauss III, J.F. (1987)J. Biol. Chem. 262, 5333–5338.
Jansen, H., Kalman, C., Birkenäger, J.C., and Hüsmann, W.C. (1980)FEBS Lett. 112, 30–34.
Jansen, H., and De Greef, W.J. (1981)Biochem. J. 196, 739–745.
Doolittle, M.H., Wong, H., Davis, R.C., and Schotz, M.C. (1987)J. Lipid Res. 28, 1326–1334.
Hixenbaugh, E.A., Sullivan, Jr., T.R., Straus III, J.F., Laposata, E.A., Komaromy, M., and Paavola, L.G. (1989)J. Biol. Chem. 264, 4222–4230.
Peterson, J., Olivecrona, T., and Bengtsson-Olivecrona, G. (1985)Biochim. Biophys. Acta 837, 262–270.
Glaser, D.S., Yost, T.J., and Eckel, R.H. (1992)J. Lipid Res. 33, 209–214.
Eckel, R.H. (1987) inLipoprotein Lipase (Borensztajn, J., ed.) pp. 79–132, Evener, Chicago.
Borensztajn, J. (1987) inLipoprotein Lipase (Borensztajn, J., ed.) pp. 133–148, Evener, Chicago.
Cherkes, A., and Gordon, Jr., R.S. (1959)J. Lipid Res. 1, 97–101.
Robinson, D.S. (1965)J. Lipid Res. 6, 222–227.
Tan, M.H., Sata, T., and Havel, R.J. (1977)J. Lipid Res. 18, 363–370.
Grinberg, D.R., Ramírez, I., Vilaró, S., Reina, M., Llobera, M., and Herrera, E. (1985)Biochim. Biophys. Acta 833, 217–222.
Galan, X., Llobera, M., and Ramírez, I. (1993)Biol. Neonate. 64, 295–303.
Ramírez, I., Kryski, A.J., Ben-Zeev, O., Schotz, M.C., and Severson, D.L. (1985)Biochem. J. 232, 229–236.
Vilaró, S., Reina, M., Ramírez, I., and Llobera, M. (1986)Biochem. J. 236, 273–278.
Burgaya, F., Peinado, J., Llobera, M., and Ramírez, I. (1989)Biosci. Rep. 9, 559–564.
Wilson, D.E., Flowers, C.M., Carlile, S.I., and Udall, K.S. (1976)Atherosclerosis 24, 491–499.
Peinado-Onsurbe, J., Soler, C., Galan, X., Poveda, B., Soley, M., Llobera, M., and Ramírez, I. (1991)Endocrinology 129, 2599–2606.
Nikkilä, E.A., Taskinen, M.R., Rehunen, S., and Harkonen, M. (1978)Metabolism 27, 1661–1670.
Taskinen, M.R., and Nikkilä, E.A. (1981)Metabolism 30, 810–817.
Taskinen, M.R., Nikkilä, E.A., Kuusi, T., and Harno, K. (1982)Diabetologia 22, 46–50.
Henderson, A.D., Richmond, W., and Elkeles, R.S. (1993)Clin. Chem. 39, 218–223.
Hamosh, M., and Hamosh, P. (1975)J. Clin. Invest. 55, 1132–1135.
Cryer, A., and Jones, H.M. (1979)Comp. Biochem. Physiol. 63B, 501–505.
Ramírez, I. (1981)Am. J. Physiol. 240, E533-E538.
Malendowicz, L.K., and Paluszak, J. (1984)Horm. Metab. Res. 16, 646–649.
Staels, B., Jansen, H., van Tol, A., Stahnke, G., Will, H., Verhoeven, G., and Auwerx, J. (1990)J. Lipid Res. 31, 1211–1218.
Tikkanen, M.J., and Nikkilä, E.A. (1987)Am. Heart J. 113, 562–567.
Applebaum, D.M., Goldberg, A.P., Pykälistö, O.J., Brunzell, J.D., and Hazzard, W.R. (1977)J. Clin. Invest. 59, 601–608.
Applebaum-Bowden, D., McLean, D., Steinmetz, A., Fontana, D., Matthys, C., Warnick, G.R., Cheung, M., Albers, J.J., and Hazzard, W.R. (1989)J. Lipid Res. 30, 1895–1906.
Tikkanen, M.J., Kuusi, T., Nikkilä, E.A., and Stenman, U.H. (1986)Metabolism 35, 99–104.
Huttunen, J.K., Ehnholm, C., Kekki, M., and Nikkilä, E.A. (1976)Clin. Sci. Mol. Med. 50, 249–260.
Eckel, R.H., and Robbins, R.J. (1984)Proc. Natl. Acad. Sci. USA 81, 7604–7607.
Gavin, L.A., Cavalieri, R.R., Moeller, M., McMahon, F.A., Castle, J.N., and Gulli, R. (1987)Metabolism 36, 919–924.
Hamosh, M., and Hamosh, P. (1975)Biochim. Biophys. Acta 380, 132–140.
Fried, S.K., Hill, J.O., Nickel, M., and Digirolamo, M. (1983)J. Nutr. 113, 1861–1869.
Studer, R.K., and Borle, A.B. (1982)J. Biol. Chem. 257, 7987–7993.
Studer, R.K., and Ganas, L. (1988)Biochim. Biophys. Acta 969, 78–85.
Guerre-Millo, M., Leturque, A., Girard, J., and Lavau, M. (1985)J. Clin. Invest. 76, 109–116.
Okamura, T., and Saito, K. (1190)Biochim. Biophys. Acta 1051, 300–305.
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Galan, X., Llobera, M. & Ramírez, I. Lipoprotein lipase and hepatic lipase in Wistar and Sprague-Dawley rat tissues. Differences in the effects of gender and fasting. Lipids 29, 333–336 (1994). https://doi.org/10.1007/BF02537186
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DOI: https://doi.org/10.1007/BF02537186