Abstract
An improved method was developed for measuring malondialdehyde (MDA) as its thiobarbituric acid (TBA) complex. Samples were initially incubated with 1% potassium iodide and 0.1% butylated hydroxytoluene at 50°C for 20 min, and then with 0.4% TBA at 60°C for 60 min. The MDA-TBA complex formed was extracted with isobutyl alcohol and measured by high-performance liquid chromatography with fluorescence detection. The improved method allows for a more specific determination of MDA present in biological samples.
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Abbreviations
- BHT:
-
butylated hydroxytoluene
- HPLC:
-
high-performance liquid chromatography
- LOH:
-
hydroxy fatty acid
- LOOH:
-
fatty acid hydroperoxide
- MDA:
-
malondialdehyde
- TBA:
-
thiobarbituric acid
- TMP:
-
1,1,3,3-tetramethoxypropane
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Li, XY., Chow, C.K. An improved method for the measurement of malondialdehyde in biological samples. Lipids 29, 73–75 (1994). https://doi.org/10.1007/BF02537094
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DOI: https://doi.org/10.1007/BF02537094