Abstract
The molecular heterogeneity of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (alkylacetyl-GPC) and 1-acyl-2-acetyl-sn-glycero-3-phosphocholine (acylacetyl-GPC) in normal rat glandular stomach was studied by gas chromatography/mass spectrometry (GC/MS) and tandem mass spectrometry. The percentage compositions of the molecular species of 1-alkyl-2-acetyl-GPC and 1-acyl-2-acetyl-GPC in the antrum were, respectively. 1-alkyl [16∶0 (34%) and 18∶0 (66%)]-2-acetyl-GPC and 1-acyl [16∶0 (60%), 18∶0 (14%) and 18∶1 (26%)]-2-acetyl-GPC. The alkyl chain composition of 1-alkyl-2-acyl-GPC was quite different from that of 1-alkyl-2-acyl-GPC in both the antrum and corpus, demonstrating a high degree of selectivity of alkyl chain utilization in PAF biosynthesis. The amount of 1-acyl-2-acetyl-GPC was much greater than that of 1-alkyl-2-acetyl-GPC. The molecular heterogeneity of 1-alkyl-2-acetyl-GPC and 1-acyl-2-acetyl-GPC in the corpus was similar to that in the antrum. Water-immersion stress affected not only the amount of 1-alkyl-2-acetyl-GPC and 1-acyl-2-acetyl-GPC, but also their molecular heterogeneity in the antrum and corpus. Whereas the amounts of 1-hexadecyl-2-acetyl-GPC and 1-acyl [16∶0, 18∶0 and 18∶1]-2-acetyl-GPC decreased markedly (to less than one-fifth) in the antrum after such stress for 1 hr, the amount of 1-octadecyl-2-acetyl-GPC increased markedly (up to 4-fold) in the corpus and severe lesions were observed after stress for 7 hr. The changes may be associated with the pathogenicity of gastric ulcers.
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Abbreviations
- GC/MS:
-
gas chromatography/mass spectrometry
- GPC:
-
sn-glycero-3-phosphocholine
- PAF:
-
platelet-activating factor
- SIM:
-
selected ion monitoring
- t-BDMS:
-
tert-butyldimethylsilyl
- TLC:
-
thin-layer chromotography
- TNS:
-
6-p-toluidine-2-naphthalenesulfonic acid
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Sugatani, J., Fujimura, K., Miwa, M. et al. Molecular heterogeneity of platelet-activating factor (PAF) in rat glandular stomach determined by gas chromatography/mass spectrometry. PAF molecular species changes upon water-immersion stress. Lipids 26, 1347–1353 (1991). https://doi.org/10.1007/BF02536564
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DOI: https://doi.org/10.1007/BF02536564