Abstract
The thiobarbituric acid (TBA) reaction was performed on linoleic acid 13-monohydroperoxide, autoxidized fatty esters, edible fats and oils, rat liver microsomal lipids, and on human erythrocyte ghost lipids in order to determine which substances from peroxidized lipids are TBA-reactive. The reaction was carried out in 2% acetic acid containing butylated hydroxytoluene using two different reaction modes: a one-step mode which involves heating at 100°C, and a two-step mode which involves first treatment at 5°C and subsequent heating at 100°C. Yields of the red 1∶2 malonaldehyde/TBA adduct, as estimated by absorbance, fluorescence intensity and high-performance liquid chromatography, were much higher than the malonaldehyde content as determined by direct chemical analysis. Yields of red pigment obtained by the two-step mode were slightly higher than those obtained by the one-step mode. Pigment yields were dramatically increased by addition oft-butyl hydroperoxide. Red pigment formation from alkenals and alkadienals was similarly enhanced by the two-step mode or by addition oft-butyl hydroperoxide, whereas pigment formation from malonaldehyde was not. It appears likely that a component of the total red pigment formed from the peroxidized lipids was due to aldehyde species other than malonaldehyde.
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Abbreviations
- TBA:
-
thiobarbituric acid
- t-BuOOH:
-
t-butyl hydroperoxide
- ADP:
-
adenosine-5′-diphosphate
- BHT:
-
butylated hydroxytoluene
- AOM:
-
active oxygen method
- HPLC:
-
highperformance liquid chromatography
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Kosugi, H., Kojima, T. & Kikugawa, K. Thiobarbituric acid-reactive substances from peroxidized lipids. Lipids 24, 873–881 (1989). https://doi.org/10.1007/BF02535762
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DOI: https://doi.org/10.1007/BF02535762