Abstract
The hydroperoxides of methyl linoleate, 1-palmitoyl-2-linoleoyl-phosphatidylcholine and trilinolein each produced similar fluorescent substances through reaction with amino compounds after decomposition by heme methyl ester. Fluorescent substances formed from methyl linoleate with 1-aminopentane revealed characeristic fluorescence peaks on HPLC, while those obtained from 1-palmitoyl-2-linoleoyl-phosphatidylcholine and trillinolein were not eluted under the same conditions. However, when both of these fluorescent substances were transesterified to methyl ester, the same fluorescence peaks were observed. This result suggests that fluorescent substances formed from oxidized membrane lipids with amino compounds remain attached to phospholipids without being released from their glycerol backbone.
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Abbreviations
- HM:
-
heme methyl ester
- HPLC:
-
high performance liquid chromatography
- HPO:
-
hydroperoxides
- ML:
-
methyl linoleate
- PLPC:
-
1-palmitoyl-2-linoleoyl-phosphatidylcholine
- PUFA:
-
polyunsaturated fatty acids
- SP:
-
secondary oxidation products
- TL:
-
trilinolein
- TLC:
-
thin layer chromatography
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Iio, T., Yoden, K. Fluorescence formation from hydroperoxide of phosphatidylcholine with amino compound. Lipids 23, 65–67 (1988). https://doi.org/10.1007/BF02535307
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DOI: https://doi.org/10.1007/BF02535307