Abstract
A simple on-line hydrogenation method in GC-MS analysis of unsaturated fatty acid esters is described. Using hydrogen as carrier gas, hydrogenation takes place in a capillary reactor connected to the outlet of the analytical column in the oven of the gas chromatograph. The reactor is a fused silica tube (60 cm×0.32 mm i.d.) coated with palladium acetylacetonate. Selective hydrogenation of olefinic bonds is achieved after a normal chromatographic run. Structural information (carbon-skeleton, double bond equivalents) can thus be deduced, and structural correlations between the saturated and unsaturated components can be obtained. Structures of cyclic fatty acid esters isolated from heated linseed oil were elucidated using this simple method which was found very useful for structural investigations on unsaturated compounds by GC-MS.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- CFAM:
-
cyclic fatty acid monomers
- GC:
-
gas chromatography
- MS:
-
mass spectroscopy
- TIC:
-
total ion currents
References
Sébédio, J.L., Prévost, J., and Grandgirard, A. (1987)J. Am. Oil. Chem. Soc. 64, 1026–1032.
Sébédio, J.L., Le Quéré, J.L., Sémon, E., Morin, O., Prévost, J., and Grandgirard, A. (1987)J. Am. Oil Chem. Soc. 64, 1324–1333.
Sébédio, J.L. Le Quéré, J.L., Morin, O., Vatèle, J.M., and Grandgirard, A. (1989)J. Am. Oil Chem. Soc., in press.
Henneberg, D., Henrichs, U., and Schomburg, G. (1975)J. Chromatogr. 112, 343–352.
Schomburg, G., Hübinger, E., Husmann, H., and Weeke, F. (1982)Chromatographia 16, 228–232.
Potteau, B., Dubois, P., and Rigaud, J. (1978)Ann. Technol. Agric. 27, 655–679.
Vatèle, J.M., Sébédio, J.L., and Le Quéré, J.L. (1988)Chem. Phys. Lipids 48, 119–128.
Author information
Authors and Affiliations
Additional information
INRA—Station de Recherches sur la Qualite des Aliments de l'Homme
About this article
Cite this article
Le Quéré, J.L., Sémon, E., Lanher, B. et al. On-line hydrogenation in GC-MS analysis of cyclic fatty acid monomers isolated from heated linseed oil. Lipids 24, 347–350 (1989). https://doi.org/10.1007/BF02535175
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02535175