Abstract
The existence of ether-linked phospholipids, including 1-O-alk-1′-enyl-2-acyl and 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholines and ethanolamines in bonitoEuthynnus pelamis (Linnaeus) white muscle, was investigated by gas chromatography and gas chromatography-mass spectrometry. Chemical ionization (iso-butane) mass spectrometry of trimethylsilyl ethers derived from the corresponding ether-linked glycerophospholipids proved effective not only for determining molecular weights but also for structural identification based on the ions [M−R]+, [M−RO]+ and [M+1]+. 1-O-Alk-1′-enyl-2-acyl-sn-glycero-3-phosphocholine and ethanolamine accounted for 3.0–6.0% and 3.6–7.6% of the total glycerophospholipids, respectively. 1-O-Alkyl-2-acyl-sn-glycero-3-phosphocholine and ethanolamine were also determined for one fish and accounted for 1.4% and 0.6% of the total glycerophospholipids, respectively. The predominant long chains in thesn-1 position of the glycerol moieties were 16∶0, 18∶0 and 18∶1 in the case of the alkenylacyl and alkylacyl components. Fatty acid distribution of individual glycerophospholipids was also determined.
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Abbreviations
- CI:
-
chemical ionization
- CPG:
-
choline glycerophospholipid
- DMA:
-
dimethylacetal
- EI:
-
electron impact
- EPG:
-
ethanolamine glycerophospholipid
- GLC:
-
gas-liquid chromatography
- LCA:
-
long chain aldehyde
- LPC:
-
lyso choline glycerophospholipid
- LPE:
-
lysoethanolamine glycerophospholipid
- MS:
-
mass spectrometry
- O:
-
origin
- PUFA:
-
polyunsaturated fatty acids
- SF:
-
solvent front
- TLC:
-
thin-layer chromatography
- TMS:
-
trimethylsilyl
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Ohshima, T., Wada, S. & Koizumi, C. 1-O-alk-1′-enyl-2-acyl and 1-O-alkyl-2-acyl glycerophospholipids in white muscle of bonitoEuthynnus pelamis (Linnaeus). Lipids 24, 363–370 (1989). https://doi.org/10.1007/BF02535142
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DOI: https://doi.org/10.1007/BF02535142