Abstract
The lymphatic absorption of nonvolatile oxidation products (NVOP) formed during heating of fats was studied. Heated colza or soybean oils or synthetic triglycerides containing a definite aromatic or alicyclic fatty acid were fed to thoracic duct-cannulated rats. Tritium-labeled triolein was added to each dietary fat, as an internal standard, in order to calculate the percentage of lymphatic absorption of the ingested NVOP. Results show that 4% of the total polymeric acids, 53% of the total oxidized monomeric acids and 96% of the total cyclic monomeric acids were recovered in the lymphatic lipids. Gas liquid and quantitative thin layer chromatography of these 3 classes indicated that, within a NVOP class, the various constituents did not present the same absorption rate. The lymphatic absorptions of individual oxidized monomers were between 25 and 93%. Concerning the polymer fraction, the lymphatic recoveries were 1% (nonpolar dimers), 6.8% (polar dimers) and 12% (polar oligomers). Aromatic acids were absorbed to a lesser degree (50–60%) than cyclohexenic acids (91–98%).
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References
Johnson, O.C., and F.A. Kummerow, J. Am. Oil Chem. Soc. 34:407 (1957).
Perkins, E.G., and L.A. Van Akkeren, J. Am. Oil Chem. Soc. 42:782 (1965).
Artman, N.R., and J.C. Alexander, J. Am. Oil Chem. Soc. 45:643 (1968).
Yasuda, K., B.R. Reddy and S.S. Chang, J. Am. Oil Chem. Soc. 45:625 (1968).
Artman, N.R., and D.E. Smith, J. Am. Oil Chem. Soc. 49:318 (1972).
Guillaumin, R., M. Gente and M. Désireux, Rev. Fr. Corps Gras 20:413 (1973).
Paulose, M.M., and S.S. Chang, J. Am. Oil Chem. Soc. 50:147 (1973).
Perkins, E.G., and L.R. Wantland, J. Am. Oil Chem. Soc. 50:459 (1973).
Risser, N., F.A. Kummerow and E.G. Perkins, Proc. Soc. Exp. Biol. Med. 121:294 (1966).
Perkins, E.G., S.M. Vachha and F.A. Kummerow, J. Nutr. 100:725 (1970).
Perkins, E.G., Rev. Fr. Corps Gras 23:313 (1976).
Hsieh, A., and E.G. Perkins, Lipids 11:763 (1976).
Guillaumin, R., Rev. Fr. Corps Gras 18:445 (1971).
Savary, P., and M.J. Constantin, Biochim. Biophys. Acta 218:195 (1970).
Sarda, L., and P. Desnuelle, Biochim. Biophys. Acta 30:513 (1958).
Brockerhoff, H., Biochim. Biophys. Acta 159: 296 (1968).
Savary, P., Biochim. Biophys. Acta 270:463 (1972).
Johnson, O.C., E.G. Perkins, M. Sugai and F.A. Kummerow, J. Am. Oil Chem. Soc. 34:593 (1957).
Karmen, A., M. Whyte and W.S. Goodman, J. Lipid Res. 4:312 (1963).
Boucrot, P., and J. Clément, C. R. Acad. Sci. 260:4083 (1965).
Savary, P., and M.J. Constantin, Biochim. Biophys. Acta 144:549 (1967).
Senior, J.R., J. Lipid Res. 5:495 (1964).
Friedrich, J.P., J. Am. Oil Chem. Soc. 44:244 (1967).
Graille, J., A. Bonfand, P. Perfetti and M. Naudet, Chem. Phys. Lipids 27:23 (1980).
Mattson, F.M., and R.A. Volpenheim, J. Lipid Res. 3:281 (1962).
Bollman, J.L., J.C. Cain and J.M. Gindley, J. Lab. Clin. Med. 33:1349 (1948).
Folch, J., M. Lees and G.H. Sloane-Stanley, J. Biol. Chem. 226:497 (1957).
Naudet, M., and S. Biasini, Rev. Fr. Corps Gras 19:307 (1972).
Méthode 2401, in “Méthodes d'Analyse des Matières Grasses et Dérivés de l'U.I.C.P.A.,” 6ème Édition, édit. Institut des Corps Gras, Paris, 1979.
Graille, J., S. Biasini and M. Naudet, Rev. Fr. Corps Gras 21:633 (1974).
Graille, J., R. Guillaumin and M. Naudet, Rev. Fr. Corps Gras 22:313 (1975).
Gente, M., and R. Guillaumin, Rev. Fr. Corps Gras 24:211 (1977).
Graille, J., F. Bedié and M. Naudet, Rev. Fr. Corps Gras 18:537 (1971).
Graille, J., and M. Naudet, Rev. Fr. Corps Gras 18:609 (1971).
Naudet, M., Rev. Fr. Corps Gras 24:482 (1977).
Ottaviani, P., J. Graille, P. Perfetti and M. Naudet, Chem. Phys. Lipids 24:57 (1979).
Artman, N.R., in “Advances in Lipid Research,” Vol. 7, Academic Press, New York, 1969, p. 245.
Michael, W.R., J.C. Alexander and N.R. Artman, Lipids 1:353 (1966).
Mattson, F.H., and R.A. Volpenhein, J. Biol. Chem. 239:2772 (1964).
Brockerhoff, H., and R.G. Jensen, in “Lipolytic Enzymes”, Academic Press, New York, 1974, p. 34.
Hofmann, A.F., and B. Borgström, Fed. Proc. 21:43 (1962).
Hofmann, A.F., and D.M. Small, Ann. Rev. Med. 18:333 (1967).
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Combe, N., Constantin, M.J. & Entressangles, B. Lymphatic absorption of nonvolatile oxidation products of heated oils in the rat. Lipids 16, 8–14 (1981). https://doi.org/10.1007/BF02534914
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DOI: https://doi.org/10.1007/BF02534914