Abstract
A two-step development procedure with partial scanning after the first development resolves seawater lipids into seven classes. The low concentration in seawater of some of these classes necessitates calibration close to the detection limit of the flame ionization detector (FID). From 0.2 to 5.0 μg the FID response usually is curvilinear, necessitating multilevel calibration. Interrod precision was poor for most of this range, and this prompted an investigation of factors affecting FID responses.
Peak areas were found to depend on the distribution of lipid material on the Chromarod. Also investigated were the effects of temperature, humidity, acid cleaning and double developments. All of these factors had some effect of Rf values, on absolute FID responses and on reproducibility, but they were not the major cause of variability in the Iatroscan-Chromarod system. Similarly, double developments improve peak shape and response, but results in only a small increase in precision for some compounds. The differences among the FID responses obtained from 10 rods within a set imply that the normalization of FID responses to that of an internal standard, or the use of intrarod rather than interrod data, should result in an increase in reproducibility. Neither of these approaches was found to improve the precision for all lipid classes. An understanding of both interrod and intrarod variability, and the use of calibration curves, is essential for accurate determination of the lipid classes of 1 liter of seawater.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Hammond, E.W. (1981) J. Chromatogr. 203, 397–403.
Parrish, C.C., and Ackman, R.G. (1983) J. Chromatogr. 262, 103–112.
Delmas, R.P., Parrish, C.C., and Ackman, R.G. (1984) Anal. Chem. 56, 1272–1277.
Crane, R.T., Goheen, S.C., Larkin, E.C., and Rao, G.A. (1983) Lipids 18, 74–80.
Parrish, C.C., and Ackman, R.G. (1983) Lipids 18, 563–565.
Ranny, M., Zbirovsky, M., Blahova, M., Ruzicka, V., and Truchik, S. (1982) J. Chromatogr. 247, 327–334.
Farnworth, E.R., Thompson, B.K., and Kramer, J.K.G. (1982) J. Chromatogr. 240, 463–474.
Petersson, B. (1982) J. Chromatogr. 242, 313–322.
Poirier, M.A., Rahimi, P., and Ahmed, S.M. (1984) J. Chromatogr. Sci. 22, 116–119.
Sebedio, J-L., and Ackman, R.G. (1984) J. Chromatogr. Sci. 19, 552–557.
Sipos, J.C., and Ackman, R.G. (1978) J. Chromatogr. Sci. 16, 443–447.
Tatara, T., Fujii, T., Kawase, T., and Minagawa, M. (1983) Lipids 18, 732–736.
Phillips, F.C., Erdahl, W.L., and Privett, O.S. (1982) Lipids 17, 992–997.
Bromly, J.H., and Roga, P. (1980) J. Chromatogr. Sci. 18, 606–613.
Kaitaranta, J.K., and Nicolaides, N. (1981) J. Chromatogr. 205, 339–347.
Harvey, H.R., and Patton, J.S. (1981) Anal. Biochem. 116, 312–316.
Mares, P., Ranny, M., Sedlacek, J., and Skorepa, J. (1983) J. Chromatogr. 275, 295–305.
Banks, F.B. (1983) J. Chromatogr. 254, 247–255.
Dallas, M.S.J. (1965) J. Chromatogr. 17, 267–277.
Ackman, R.G., and Woyewoda, A.D. (1979) J. Chromatogr. Sci. 17, 514–517.
Keith, L.H., Crummett, W., Deegan, J., Libby, R.A., Taylor, J.K., and Wentler, G. (1983) Anal. Chem. 55, 2210–2218.
Patterson, P.L. (1985) Lipids 20, XXX-XXX.
Read, H. (1985) Lipids 20, XXX-XXX.
Whitehouse, B.G., Parrish, C.C., and Wangersky, P.J. (1984) The Analysis of Organic Compounds in Seawater, 1978–1983, Mar. Anal. Chem. Stand. Prog., Rep. 9, Nat. Res. Coun. Can. No. 23609.
Author information
Authors and Affiliations
About this article
Cite this article
Parrish, C.C., Ackman, R.G. Calibration of the Iatroscan-Chromarod system for marine lipid class analyses. Lipids 20, 521–530 (1985). https://doi.org/10.1007/BF02534893
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02534893