Abstract
The analysis of triglyceride species by high performance liquid chromatography (HPLC) with a flame ionization detector (FID) and reversed-phase chromatography using chemically bonded octadecyl silane (ODS) Zorbax columns and gradient or isocratic solvent elution with methylene chloride/acetonitrile is described. Triglycerides containing acyl groups of critical pairs,trans and positional isomers, as well as mixtures of even and odd chain lengths are separated. Identification of triglycerides is made on the basis of retention times compared with equivalent and theoretical carbon numbers, and comparison with chromatograms of reference triglyceride mixtures. The methodology is demonstrated by fractionizing the triglycerides of olive oil under different chromatographic conditions using single and coupled conventional 250×4.6 mm columns and a short 80×6.2 mm column for fast separations.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Nickell, E.C., and Privett, O.S. (1967) Sep. Sci 2, 307–318.
Pei, P., Henley, R., and Ramachandran, S. (1975) Lipids 10, 152–156.
Plattner, R.D., Spencer, G.F., and Kleiman, R. (1977) J. Am. Oil Chem. Soc. 54, 511–515.
Plattner, R.D. (1981) Methods Enzymol. 72, 21–34.
El-Hamdy, A.H., and Perkins, E.G. (1981) J. Am. Oil Chem. Soc. 58, 867–872.
Perkins, E.G., Hendren, D.J., Pelick, N., and Bauer, J.E. (1982) Lipids 17, 460–463.
Dong, M.W., and DiCesare, J.L. (1983) J. Am. Oil Chem. Soc. 60, 788–791.
Stolyhwo, A., and Privett, O.S. (1973). J. Chromatogr. Sci. 11, 20–25.
Erdahl, W.L., Stolyhwo, A., and Privett, O.S. (1973) J. Am. Oil Chem. Soc. 50, 513–515.
Privett, O.S., Dougherty, K.A., Erdahl, W.L., and Stolyhwo, A. (1973) J. Am. Oil Chem. Soc. 50, 516–520.
Phillips, F.C., Erdahl, W.L., and Privett, O.S. (1982) Lipids 17, 992–97.
Phillips, F.C., and Privett, O.S. (1981) J. Am. Oil Chem. Soc. 58, 590–594.
Privett, O.S., and Erdahl, W.L. (1978) Anal. Biochem. 84, 449–461.
Christie, W.W. (1982) J. Lipid Res. 23, 1072–1075.
Snyder, L.R., Dolan, J.W., and Grant, J.R. (1979) J. Chromatogr. Chromatogr. Rev. 165, 3–30.
Kuksis, A., Marai, L., and Myher, J.J. (1983) J. Chromatogr. 273, 43–66.
Privett, O.S., and Erdahl, W.L. (1981) Methods Enzymol. 72, 56–108.
Author information
Authors and Affiliations
Additional information
An erratum to this article is available at http://dx.doi.org/10.1007/BF02534459.
About this article
Cite this article
Phillips, F.C., Erdahl, W.L., Nadenicek, J.D. et al. Analysis of triglyceride species by high-performance liquid chromatography via a flame lonization detector. Lipids 19, 142–150 (1984). https://doi.org/10.1007/BF02534506
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02534506