Abstract
A colorimetric microassay is described for the determination of lipid hydroperoxides. Hydroperoxides are reacted with potassium iodide in the presence of an acid catalyst and liberated iodine is measured. Aluminum chloride, an alcohol-soluble Lewis acid, is used as catalyst. Liberated iodine is measured colorimetrically at 560 nm after addition of starch in 0.01 N hydrochloric acid. The range of the measurement was 0.05–0.5 μmol of hydroperoxides.
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Asakawa, T., Matsushita, S. A colorimetric microdetermination of peroxide values utilizing aluminum chloride as the catalyst. Lipids 15, 965–967 (1980). https://doi.org/10.1007/BF02534423
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DOI: https://doi.org/10.1007/BF02534423