Abstract
The membranes of yeast mitochondria were separated and analyzed for lipid content. The sterolto-phospholipid molar ratio was found to be very similar between the inner and outer membranes (1∶30). These observed ratios could be substantially altered by using a crude mitochondrial pellet contaminated with a “floating lipid layer”. In this case, the sterol-to-phospholipid molar ratios were 1∶8 to 1∶26 for the outer and inner mitochondrial membranes, respectively.
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Bottema, C.K., Parks, L.W. Sterol analysis of the inner and outer mitochondrial membranes in yeast. Lipids 15, 987–992 (1980). https://doi.org/10.1007/BF02534312
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DOI: https://doi.org/10.1007/BF02534312