Abstract
Open tubular gas liquid chromatographic (GLC) analyses of fatty acids from total lipids of 12 species of Echinoidea collected at several locations along the Pacific coast of Japan showed the same unusualcis-5-olefinic acids in all species, i.e.,cis-5-octadecenoic acid (5–18∶1),cis-5-eicosenoic acid (5–20∶1), all-cis-5,11- and 5,13-eicosadienoic acids (5,11- and 5,13–20∶2), allcis-5,11,14-eicosatrienoic acid (5,11,14–20∶3) and all-cis-5,11,14,17-eicosatetraenoic acid (5,11,14,17–20∶4). The structural analysis of partially purified 5,11,14,17–20∶4 was undertaken by reductive ozonolysis with GLC and gas chromatographic-mass spectrometric analyses of the products.13C-Nuclear magnetic resonance analyses of the totals and fractions of fatty acid methyl esters from the sea urchin lipids did not show any occurrence of fatty acids having an isolated olefinic bond in the 2, 3 or 4 positions. The 5-olefinic acids were concentrated on the polar lipids rather than neutral lipids. The branched and odd chain fatty acid contents of mud-feeding sea urchins were found to be relatively greater proportions of total fatty acids than in algae feeders.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Takagi, T., Eaton, C.A., and Ackman, R.G. (1980)Can. J. Fish. Aquat. Sci. 37, 195–202.
Itabashi, Y., and Takagi, T. (1980)Yukagaku 29, 855–865.
Takagi, T., Kaneniwa, M., and Itabashi, Y. (1982)Bull. Fac. Fish, Hokkaido Univ. 33, 263–269.
Takagi, T., and Itabashi, Y. (1982)Lipids 17, 716–723.
Bligh, E.G., and Dyer, W.J. (1959)Can. J. Biochem. Physiol. 37, 911–917.
Kleiman, R., Spencer, G.F., Earle, F.R., and Wolff, I.A. (1969)Lipids 4, 135–141.
Ackman, R.G. (1964)J. Gas Chromatogr. 2, 173–179.
Kochi, M. (1976)J. Shimonoseki Univ. Fish. 25 83–153.
Kochi, M. (1976)Bull. Jpn. Soc. Sci. Fish. 42 721–729.
Bus, J., Sies, I., and Lie Ken Jie, M.S.F. (1977)Chem. Phys. Lipids 18, 130–144.
Christie, W.W. (1982)Lipid Analysis, 2nd edn., pp. 79, Pergamon Press, Oxford.
Ackman, R.G., and Hooper, S.N. (1973)Comp. Biochem. Physiol. 46B, 153–165.
Paradis, M., and Ackman, R.G. (1977)Lipids 12, 170–176.
Paradis, M., and Ackman, R.G. (1975)Lipids 10, 12–16.
Takagi, T., Hayashi, K., and Itabashi, Y. (1981)Proc. Jpn. Conf. Biochem. Lipids 23, 254–257.
Ackman, R.G., and McLachan, J. (1977)Proc. N. S. Inst. Sci. 28, 47–64.
Johns, R.B., and Nicholes, P.D. (1979)Phytochemistry 18, 799–802.
Lawrence, J.M. (1975)Oceanogr. Mar. Biol. Ann Rev. 13, 213–286.
Hayashi, K., and Takagi, T. (1977)Bull. Fac. Fish. Hokkaido Univ. 28, 40–46.
Sen, N., and Schlenk, H. (1964)J. Am. Oil Chem. Soc. 41, 241–248.
Deng, J.C., Orthoefer, F.T., Dennison, R.A., and Watson, M. (1976)J. Food Sci. 41, 1479–1483.
Addison, R.F., and Ackman, R.G. (1970)Lipids 5, 554–557.
Lewis, R.W. (1967)J. Fish. Res. Board Can. 24, 1101–1115.
Leo, R.F., and Parker, P.L. (1966)Science 152, 649–650.
Ratnayake, W.N., and Ackman, R.G. (1979)Lipids 14, 580–584.
Ackman, R.G. (1981) inNew Sources of Fats and Oils (Pryde, E.H., Princen, L.H., and Mukherjee, K.D., eds.) pp. 189–220, American Oil Dhemists' Society, Champaign, IL.
Pollard, M.R., and Stumpf, P.K. (1980)Plant Physiol. 66, 649–655.
Moreau, R.A., Pollard, M.R., and Stumpf, P.K. (1981)Arch. Biochem. Biophys. 209, 376–384.
Klingensmith, J.S. (1982)Lipids 17, 976–981.
Irazu, C.R., Pollerro, R.J., and Brenner, R.R. (1984)Lipids 19, 649–655.
Morales, R.W., and Litchfield, C. (1977)Lipids 12, 570–576.
Walkup, R.D., Jamieson, G.C., Ratcliff, M.R., and Djerassi, C. (1981)Lipids 16, 631–646.
Isay, S.V., and Busarova, N.G. (1984)Comp. Biochem. Physiol. 77B, 803–810.
Author information
Authors and Affiliations
About this article
Cite this article
Takagi, T., Kaneniwa, M., Itabashi, Y. et al. Fatty acids in echinoidea: Unusualcis-5-olefinic acids as distinctive lipid components in sea urchins. Lipids 21, 558–565 (1986). https://doi.org/10.1007/BF02534052
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02534052