Abstract
The objective of this study was to follow the uptake and distribution of oleic and elaidic acids into human erythrocytes, platelets, and plasma phospholipids. The use of dual and triple labeling methodology permitted a precise comparison of elaidic and oleic acid utilization. Elaidic acid (EI) was selectively concentrated in all the plasma phospholipids except for lysophosphatidylcholine. Three times more elaidic than oleic acid (Ol) accumulated in the 1-acyl position of phosphatidylcholine, as determined by hydrolysis with phospholipase A2. Rapid incorporation and removal of elaidate were observed for all samples. These results support the concept that enzymes responsible for acylation of phospholipids are sensitive to double bond configuration and the physical properties of the fatty acid moieties. Labeled fatty acid levels in red cell and platelet phospholipids were much lower than for plasma phospholipids, indicating a relatively slow rate for the in vivo incorporation of fatty acids into blood cell membrane phospholipids. No isotope effect was found when oleic acid labeled with deuterium on the double bond was used.
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Economic Research Service, USDA, Fats and Oil Situation 281:Feb. (1976).
Scholfield, C.R., V.L. Davision, and H.J. Dutton, J. Am. Oil Chem. Soc. 44:648 (1967).
Miljanich, P., and R. Ostwald, J. Am. Diet. Assoc. 56:29 (1970).
Carpenter, D.L., J. Lehman, B.S. Mason, and H.T. Slover, J. Am. Oil Chem. Soc. 53:713 (1976).
Pelloquin, A., and E. Ucciami, Rev. Fr. Corps. Gras. 22:379 (1975).
Nazir, D.J., B.J. Moorecroft, and M.A. Mishkel, Am. J. Clin. Nutr. 29:331 (1976).
Sreenivasan, B., and J.B. Brown, J. Am. Oil Chem. Soc. 33:341 (1956).
Kurucz-Lusztig, E., P. Lukacs-Hagony, M. Prepostffy-Janoshegyi, M. Jeranek-Knapecz, and P. Biacs, Acta Alimentaria 3:357 (1974).
Johnston, P.V., O.C. Johnson, and F.A. Kummerow, J. Nutr. 65:13 (1958).
Elson, C.E., N.J. Benevenga, D.J. Canty, R.H. Gummer, A.E. Johnston, J.J. Lalich, J.W. Portor, and E.S. Shrago, J. Am. Oil Chem. Soc. 53:468A (1976).
Johnston, P.V., O.C. Johnson, and C.H. Walton, Proc. Soc. Exp. Biol. Med. 99:735 (1958).
Mounts, T.L., E.A. Emken, W.K. Rohwedder, and H.J. Dutton, Lipids 6:912 (1971).
Mounts, T.L., Lipids 11:676 (1976).
Lanser, A.C., T.L. Mounts, and E.A. Emken, Lipids 13:103 (1978).
Emken, E.A., W.K. Rohwedder, H.J. Dutton, R. Dougherty, J.M. Iacono, and J. Mackin, Lipids 11:135 (1976).
Anderson, R.L., C.S. Fullmer, Jr., and E.J. Hollenback, J. Nutr. 105:393 (1975).
Decker, W.J., and W. Mertz, J. Nutr. 91:324 (1967).
Decker, W.J., and W. Mertz, J. Nutr. 89:165 (1966).
Searcey, M.T., and D.A. Arata, J. Nutr. 102:1429 (1972).
Wood, R., and F. Chumbler, J. Am. Oil Chem. Soc. 53:148A (1976).
Ono, K., and D.S. Fredrickson, J. Biol. Chem. 239:2482 (1964).
Weigensberg, B.I., and G.C. McMillan, J. Nutr. 83:314 (1964).
Vandenhoff, G., F.D. Gunstone, J. Barve, and W.E.M. Lands, J. Biol. Chem. 250:8720 (1975).
Baldassare, J.J., K.B. Rhinehart, and D.F. Silbert, Biochemistry 15:2986 (1976).
Nishihara, M., K. Kimura, K. Izui, M. Ishinaga, M. Kato, and M. Kito, Biochim. Biophys. Acta 409:212 (1975).
Esfahani, M., T. Ioneda, and S.J. Wakil, J. Biol. Chem. 246:50 (1971).
Esfahani, M., E.M. Barnes, Jr., and S.J. Wakil, Biochemistry 64:1057 (1969).
Vergroesen, A.J., Proc. Nutr. Soc. 31:323 (1972).
Anderson, J.T., F. Grande, and A. Keys, J. Nutr. 75:388 (1961).
DeIongh, H., R.K. Beerthuis, C. den Hartog, L.M. Dalderupond, and P.A.F. Van der Spek, Bibl. Nutr. Dieta 7:137 (1965).
McOsker, D.E., F.H. Mattson, H.B. Sweringen, and A.M. Kligman, J. Am. Med. Assoc. 180:380 (1962).
Mishkel, M.A., and N. Spritz, in “Advances in Experimental Medicine and Biology, Vol. 4, Edited by W.L. Holmes, L.A. Carlson, and K. Paoletti, Plenum Press, New York, 1969, pp. 355–364.
Alfin-Slater, R.B., R.S. Morris, H. Hansen, and J.F. Proctor, J. Nutr. 87:168 (1965).
Selinger, Z., and R.T. Holman, Biochim. Biophys. Acta 106:56 (1965).
DeTomas, M.E., R.R. Brenner, and R.O. Peluffo, Biochim. Biophys. Acta 70:472 (1963).
Egwin, P.O., and D.S. Sgoutas, J. Nutr. 101:307 (1971).
Yeh, S.C., T. Mizuguchi, and F.A. Kummerow, Proc. Soc. Exp. Biol. Med. 146:236 (1974).
McMillan, G.C., M.D. Silver, and B.I. Weigensberg, Arch. Pathol. 76:106 (1963).
Weigensberg, B.I., G.C. McMillan, and A.C. Ritchie, Arch. Pathol. 72:126 (1961).
Guo, L.S.S., and J.C. Alexander, Biochim. Biophys. Acta 369:411 (1974).
Alfin-Slater, R.B., A.F. Wells, L.A. Aftergood, and H.J. Deuel, Jr., J. Nutr. 63:241 (1957).
Gottenbos, J.J., and H.J. Thomasson, Bibl. Nutr. Dieta 7:110 (1965).
LeBreton, E., and P. Lemarchal, Cah. Nutr. Diet. 4 (suppl. 2):11 (1969).
Alfin-Slater, R.B., P. Wells, and L.A. Aftergood, J. Am. Oil Chem. Soc. 47:Abstr. 274 (1970).
Vles, R.O., and J.J. Gottenbos, Voeding 33:428 (1972).
Vles, R.O., and J.J. Gottenbos, Voeding 33:455 (1972).
DeJarlais, W.J., and E.A. Emken, Lipids 11:594 (1976).
Emken, E.A., Metabolism 28:575 (1979).
Peter, H.W., and H.U. Wolf, J. Chromatogr. 82:15 (1973).
Skipski, V.P., J.J. Good, M. Barclay, and R.B. Reggio, Biochim. Biophys. Acta 152:10 (1968).
Christie, W.W., in “Lipid Analysis,” Ch. 4, Pergamon Press, New York, 1975, pp. 85.
Robertson, A.F., and W.E.M. Lands, Biochemistry 1:804 (1962).
Rohwedder, W.K., W.F. Kwolek, D.J. Wolf, and W.L. Everhart, Biomed. Mass Spectrom. 6:67 (1979).
Okuyama, H., W.E.M. Lands, F.D. Gunstone, and J.A. Barve, Biochemistry 11:4392 (1972).
Schrock, C.G., and William E. Connor, Am. J. Clin. Nutr. 28:1020 (1975).
Mulder, E., and L.L.M. Van Deenen, Biochim. Biophys. Acta 106:106 (1965).
Winterbourn, C.C., and R.D. Batt, Biochim. Biophys. Acta 202:9 (1970).
Sakagami, T., O. Minari, and T. Orii, Biochim. Biophys. Acta 98:356 (1965).
Neerhourt, R.C., J. Lab. Clin. Med. 71:438 (1968).
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Emken, E.A., Rohwedder, W.K., Dutton, H.J. et al. Incorporation of deuterium-labeledcis- andtrans-9-octadecenoic acids in humans: Plasma, erythrocyte, and platelet phospholipids. Lipids 14, 547–554 (1979). https://doi.org/10.1007/BF02533530
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DOI: https://doi.org/10.1007/BF02533530