Abstract
Two simplified methods for quantitative lipoprotein analysis have been calibrated and compared with each other using analytic ultracentrifugation as a standard reference procedure. The first method was the Friedewald procedure and the second was an automated agarose gel electrophoresis system. Both procedures offer comparable quantitative lipoprotein analysis with potential for large scale screening purposes at low cost ($4.00–$5.00 per analysis). There were advantages and limitations to both procedures. The Friedwald procedure can be used on frozen sera but requires 3 ml sera. In contrast, the electrophoresis system must be used with fresh serum but requires only 50 μl serum and the electrophoretic slides may be quantitatively analyzed several years retrospectively.
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References
Fredrickson, D.S., R.I. Levy, and F.T. Lindgren, J. Clin. Invest. 47:2446 (1968).
Miller, G.J., and N.E. Miller, Lancet 1:16 (1975).
Manual of Laboratory Operations, Lipid Research Clinic Program, Vol. 1, Lipid and Lipoprotein Analysis, DHEW Pub. No. (NIH) pp. 75–628, Washington, DC, U.S. Government Printing Office, 1974.
Friedewald, W.T., R.I. Levy, and D.S. Fredrickson, Clin. Chem. 18:499 (1972).
Wong, R.A., P.G. Banchero, L.C. Jensen, S.S. Pan, G.L. Adamson, and F.T. Lindgren, J. Lab. Clin. Med. (in press, 1977).
The Walnut Creek Contraceptive Drug Study. A Prospective Study of the Side Effects of Oral Contraceptives, Edited by S. Ramcharan, Vol. 1, DHEW Pub. No. (NIH) pp. 75–628, Washington, DC, U.S. Government Printing Office, 1974.
Lindgren, F.T., G.L. Adamson, L.C. Jensen, and P.D. Wood, Lipids 10:750 (1975).
Stern, M.P., B.W. Brown, W.L. Haskell, J.W. Farquhar, C.L. Wehrle, and P.S. Wood, J. Am. Med. Assoc. 235:811 (1976).
Auto Analyzer II-24, May, 1971, Simultaneous Cholesterol/Triglycerides, Techicon Instruments Corp., Tarrytown, NY 10591.
Hatch, F.T., F.T. Lindgren, G.L. Adamson, L.C. Jensen, A.W. Wong, and R.I. Levy, J. Lab. Clin. Med. 81:946 (1973).
Lindgren, F.T., L.C. Jensen, and F.T. Hatch, in “Blood Lipids and Lipoproteins,” Edited by G.J. Nelson, John Wiley-Interscience, New York, NY, 1972, pp. 181–274.
Samson, E.J., D.D. Derek, and L.M. Demers, Clin. Chem. 22:346 (1976).
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Lindgren, F.T., Silvers, A., Jutagir, R. et al. A comparison of simplified methods for lipoprotein quantification using the analytic ultracentrifuge as a standard. Lipids 12, 278–282 (1977). https://doi.org/10.1007/BF02533347
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DOI: https://doi.org/10.1007/BF02533347