Abstract
The Lp(a) lipoprotein was purified from human plasma by ultracentrifugation and gel filtration on 6% agarose. It contained 27% protein, 65% lipid, and 8% carbohydrate. Quantification of the Lp(a) lipoprotein was performed by radial immunodiffusion. Both within-assay and between-assay coefficients of variation were inversely concentration dependent, decreasing from 20% and 27%, respectively, at 3 mg/100 ml to 7% and 12%, respectively, at concentrations above 8 mg/100 ml. The lower limit of sensitivity of the assay was 1.5 mg/100 ml. Of 340 unrelated fasting subjects tested, 81% had levels of the Lp(a) lipoprotein exceeding this lower limit. The distribution of Lp(a) concentrations in this population was skewed with a mean of 14 mg/100 ml and a median of 8 mg/100 ml. Lp(a) lipoprotein was not significantly correlated with age, sex, or cholesterol or glyceride concentrations.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Simons, K., C. Ehnholm, O. Renkonen and B. Bloth, Acta Pathol. Microbiol. Scand., Sec. B 78:459 (1970).
Albers, J.J., C. Chen and F. Aladjem, Biochemistry 11:57 (1972).
Ehnholm, C., H. Garoff, O. Renkonan and K. Simons, Ibid. 11:3229 (1972).
Rider, A.K., R.I. Levy, and D.S. Fredrickson, Circulation 42:III (1970).
Berg, K., Acta Pathol. Microbiol. Scand. 59:369 (1963).
Berg, K., Vox Sang. 11:419 (1966).
Harvie, N.R., and J.S. Schultz, Proc. Nat. Acad. Sci. 66:99 (1970).
Havel, R.J., H.A. Eder and J.H. Bragdon, J. Clin. Invest. 34:1345 (1955).
Narayan, K.A., H.L. Creinin and F.A. Kummerow, J. Lipid Res. 7:150 (1966).
Albers, J.J., and S. Dray, Biochem. Genet. 2:25 (1968).
Albers, J.J., and F. Aladjem, Biochemistry 10:3436 (1971).
Folch, J.M. Lees, and G.H. Sloane-Stanley, J. Biol. Chem. 226:497 (1957).
Searcy, R.L., L.M. Bergquist and R.C. Jung, J. Lipid Res. 1:349 (1960).
Carlson, L.A., Acta Med. Soc. Ups. 64:208 (1959).
Bartlett, G.R., J. Biol. Chem. 234:466 (1959).
Laboratory Methods Committee, “Manual of Laboratory Methods, Lipid Research Clinics Project,” Vol. 2, 1972.
“AutoAnalyzer Manual,” Technicon Instruments Corporation, Tarrytown, N.Y., 1970.
Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, J. Biol. Chem. 193:265 (1951).
Chase, M.W., and C.A. Williams in “Methods of Immunology and Immunochemistry,” Academic Press, New York, N.Y., 1968, p. 257–270.
Mokrasch, C., J. Biol. Chem. 208:55 (1954).
Albers, J.J., and S. Dray, J. Immunol. 103:155 (1969).
Mancini, G., A.O. Carbonara and J.F. Heremans, Int. J. Immunochem. 2:235 (1965).
Albers, J.J., and A.M. Scanu, Biochem. Biophys. Acta. 236:29 (1971).b
Hatch, F.K., and R.S. Lees, Advan. Lipid Res. 6:1 (1968).
Schrott, H.G., J.L. Goldstein, W.R. Hazzard, M.M. McGoodwin, and A.G. Motulsky, Ann. Intern. Med. 76:711 (1972).
Author information
Authors and Affiliations
About this article
Cite this article
Albers, J.J., Hazzard, W.R. Immunochemical quantification of human plasma Lp(a) lipoprotein. Lipids 9, 15–26 (1974). https://doi.org/10.1007/BF02533209
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02533209