Abstract
The long chain triglycerides of bovine milk fat were isolated by thin layer chromatography, and their chemical structure determined by combined thin layer and gas liquid chromatography, and a stereospecific analysis of a molecular distillate of butteroil of comparable composition. The milk fat fraction (39% of total) contained C8–C20 fatty acids which were distributed among the glycerides of 40–56 acyl carbon atoms in a manner not unlike that found for the same acids in the short chain triglycerides. Although individual triglycerides were not identified, the specific distribution of the fatty acids could best be accounted for by assuming a common pool of long chain 1,2-diglyceride precursors from which the bulk of both short and long chain triglycerides are synthesized by a stereospecific introduction of C4–C18 fatty acids in position 3 of sn-glycerol. This hypothesis is compatible with the results of stereospecific analyses of the short and long chain fractions and of the total butteroil. It is supported by the nonrandom distributions demonstrated for the molecular weights of the milk fat triglycerides of different degrees of saturation.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Garton, G. A., J. Lipid Res.4, 237–259 (1963).
McCarthy, R. D., S. Patton and L. Evans, J. Dairy Sci.43, 1196–1201 (1960).
Patton, S., R. D. McCarthy, L. Evans, R. G. Jensen and G. W. Gander, J. Dairy Sci.45, 248–249 (1962).
Patton, S. and R. D. McCarthy, Ibid.46, 916–921 (1963).
Boudreau, A. and J. M. Deman, Biochim. Biophys. Acta98, 47–52 (1965).
Patton, S., R. O. Mumma, R. D. McCarthy, “An Active Role of Lecithin in the Synthesis of Milk Fat”, in Abstracts of Papers, 40th Fall Meeting of the AOCS, Philadelphia, October, 1966.
Nutter, L. J. and O. S. Privett, J. Dairy Sci.50, 1194–1199 (1967).
Breckenridge, W. C. and A. Kuksis, Lipids,3, 291–300 (1968).
Pitas, R. E., J. Sampugna and R. G. Jensen, J. Dairy Sci.50, 1332–1336 (1967).
Breckenridge, W. C. and A. Kuksis, J. Lipid Res.9, 388–393 (1968).
Kuksis, A., M. J. McCarthy and J. M. R. Beveridge, J.A.O.C.S.40, 530–535 (1963).
McCarthy, M. J., A. Kuksis and J. M. R. Beveridge, Can. J. Biochem. Physiol.40, 1693–1703 (1962).
Blank, M. L. and O. S. Privett, J. Dairy Sci.57, 481–488 (1964).
Author information
Authors and Affiliations
About this article
Cite this article
Breckenridge, W.C., Kuksis, A. Structure of bovine milk fat triglycerides: II. Long chain lengths. Lipids 4, 197–204 (1969). https://doi.org/10.1007/BF02532629
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02532629