Abstract
WhenChlorella emersonii, a green alga, was cultured in the presence of 20 ppm AY-9944, a number of sterols accumulated which appear to be intermediates of sterol biosynthesis in this organism. The sterols isolated include 14α-methyl-ergost-8-en-3β-ol, 14α-methyl 24S-stigmast-8-en-3β-ol, 14α-methyl ergosta-8,24(28)-dien-3β-ol and 4α, 14α-dimethyl 24S-stigmast-8-en-3β-ol. Smaller quantities of several other sterols were found in addition to the normally occurring Δ7, chondrillasterol and Δ7. Control cultures were found to contain, in addition to the normally occurring sterols, smaller quantities of most of the sterols isolated from AY-9944 inhibited cultures. AY-9944 is a specific inhibitor of Δ7 in cholesterol biosynthesis in animals. However, sinceC. emersonii terminates sterol biosynthesis one step prior to the Δ7 step, AY-9944 apparently inhibits sterol biosynthesis prior to this step in this organism. The accumulation of 14α-methyl sterols in treated cultures suggests that AY-9944 is an effective inhibitor of the 14α-methyl removal inC. emersonii.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Dempsey, M., Ann. New York Acad. Sci. 148:631 (1968).
Dvornik, D., M. Kraml and J. Bagli, J. Amer. Chem. Soc. 86:2739 (1964).
Kraml, M., J. Bagli and D. Dvornik, Biochem. Biophys. Res. Comm. 15:455 (1964).
Dickson, L.G., G.W. Patterson, C.F. Cohen and S.R. Dutky, Phytochem. 11:3473 (1972).
Dickson, L.G., and G.W. Patterson, Lipids 7:635 (1972).
Patterson, G.W., Plant Physiol. 42:1457 (1967).
Doyle, P.J., G.W. Patterson, S.R. Dutky and M.J. Thompson, Phytochemistry 11:1951 (1972).
Patterson, G.W., Anal. Chem. 43:1165 (1971).
Author information
Authors and Affiliations
Additional information
Scientific Article No. A1865, Contribution No. 4775 of the Maryland Agricultural Experiment Station.
About this article
Cite this article
Dickson, L.G., Patterson, G.W. AY-9944 inhibition of sterol biosynthesis inChlorella emersonii . Lipids 8, 443–445 (1973). https://doi.org/10.1007/BF02531761
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02531761