Abstract
Sterols constitute 1.95% of the total extractable lipids ofAcheta domesticus L., of which 18% are esterified. The free sterols consist of cholestane-3β-ol (0.5%), Δ5-cholestene-3β-ol (83.5%), Δ7-cholestene-3β-ol (2.3%) Δ5,7-cholestadiene-3β-ol (3%), Δ5,22-cholestadiene-3β-ol (4%), Δ5,7,22-cholestatriene-3β-ol (0.2%), campestane-3β-ol (0.03%), Δ5-campestene-3β-ol (1.0%), Δ7-campestene-3β-ol (trace), Δ5,7-campestadiene-3β-ol (0.2%), stigmastane-3β-ol (0.09%), Δ5-stigmastene-3β-ol (2.1%), Δ7-stigmastene-3β-ol (0.04%), Δ5,7-stigmastadiene-3β-ol (0.4%), Δ5,22-stigmastadiene-3βol (0.1%). The same sterols are present in the esterified sterol fraction. Δ7-Sterols and Δ5,7-sterols are present in significantly larger amounts in the esterified fraction than in the free sterol fraction. By a comparison with the sterols of the cricket food, it is clear thatA. domesticus is capable of removing methyl and ethyl groups from C-24 of sterols of the campestane and stigmastane type. The ability to introduce a Δ7 double bond into saturated and Δ5-sterols is indicated, and it is suggested that Δ7-sterols of the C27, C28, and C29 sterol series may be intermediates in the conversion of Δ5-sterols to Δ5,7-sterols.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bloch, K., R. G. Langdon, A. J. Clark and G. Fraenkel, Biochim. Biophys. Acta21, 176 (1956).
Clark, A. J. and K. Block, J. Biol. Chem.234, 2589–2594 (1959).
Clayton, R. B. and A. M. Edwards, Biochem. Biophys. Research Commun.6, 281–4 (1961).
Kodicek, E. and Z. H. Levinson, Nature188, 1023–1025 (1960).
Kaplanis, J. N., R. C. Dutky and W. E. Robbins, Ann. Entomol. Soc. Am.54, 114–116 (1961).
Sedee, P. D. J. W., Arch. Intern. Physiol. Biochim.69, 284–294 (1961).
Robbins, W. E., J. N. Kaplanis, S. J. Louloudes and R. E. Monroe, Ann. Entomol. Soc. Am.53, 128–129 (1960).
Lasser, N. L., A. M. Edwards and R. B. Clayton, J. Lipid Res.7, 403–412 (1968).
Lasser, N. L. and R. B. Clayton, Ibid.7, 413–421 (1968).
Clayton, R. B., Ibid.5, 3–19 (1964).
Gilby, A. R., Ann. Rev. Entomol.10, 141–160 (1965).
Kaplanis, J. N., W. E. Robbins and L. A. Tabor, Ann. Entomol. Soc. Am.53, 260–264 (1960).
Ishii, S. I., J. N. Kaplanis and W. E. Robbins, Ibid.56, 115–119 (1963).
Schaefer, C. H., J. N. Kaplanis and W. E. Robbins, J. Insect Physiol.11, 1013–1021 (1965).
Levinson, Z. H., Ibid.8, 191–198 (1962).
Fraenkel, G. and M. Blewett, Biochem. J.37, 692–695 (1943).
Head, W. B. and H. W. Kirchner, Science149, 758–761 (1965).
Ito, T., Nature191, 882–883 (1961).
Duperon, P., M. F. Huegel, Z. Sipal and M. Barbier, Comp. Biochem. Physiol.11, 257–259 (1964).
Hutchins, R. F. N. and M. M. Martin, Lipids,3, 247–249, 1968.
Schoenheimer, R. and H. Dam, Z. Physiol. Chem.215, 59–63 (1933).
Bergmann, W., J. Biol. Chem.132, 471–472 (1940).
Rosenfeld, R. S., Anal. Biochem.12, 483–487 (1963).
Blondin, G. A., B. D. Kulkarni, J. P. John, R. T. van Aller, P. T. Russell and W. R. Nes, Anal. Chem.39, 36 (1967).
Windaus, A., and J. Brunken, Ann.460, 225 (1928).
Robbins, W. E., M. J. Thompson, J. N. Kaplanis and T. J. Shortino, Steroids4, 635–644 (1964).
Svoboda, J. A., M. J. Thompson and W. E. Robbins, Life Sciences6, 395–404 (1967).
Meinwald, J. and Y. C. Meinwald, J. Am. Chem. Soc.88, 1305–1310 (1968).
Clayton, R. B. and A. M. Edwards, Federation Proc.21, 297 (1962).
Clayton, R. B. and A. M. Edwards, J. Biol. Chem.,238, 1968–1972 (1963).
Louloudes, S. J., M. J. Thompson, R. E. Monroe and W. E. Robbins, Biochem. Biophys. Res. Commun.8, 104 (1962).
Unpublished work of F. J. Ritter, R. B. Clayton and K. Bloch cited by Clayton in Ref. ——.
Monroe, R. E., T. L. Hopkins and S. A. Valder, J. Insect Physiol.13, 219 (1967).
Clayton, R. B. and K. Bloch, J. Biol. Chem.235, 3421–3425 (1960).
Author information
Authors and Affiliations
Additional information
Associate Professor, Department of Chemistry, University of Michigan, Ann Arbor, Mich.; Alfred P. Sloan Foundation Fellow, 1968–68.
Public Health Service Predoctoral Fellow, 1968–67.
About this article
Cite this article
Martin, M.M., Carls, G.A. The lipids of the common house cricket,Acheta domesticus L. III. Sterols. Lipids 3, 256–259 (1968). https://doi.org/10.1007/BF02531197
Issue Date:
DOI: https://doi.org/10.1007/BF02531197