Abstract
Triacylglycerol is accumulated byStreptomyces spp. when grown in submerged culture. Ultrastructural studies using transmission electron microscopy (TEM), staining and freeze-fracture/freeze-etch procedures, and light microscopy confirmed the accumulation of neutral lipid byS. lividans andS. coelicolor during the stationary phase and its storage within membrane-bound globular structures within the cytoplasm. There structures were of various sizes and occupied up to approximately 80% of the total cell volume at that time. There was no evidence of such material within cells examined during the early exponential phase of growth. The globules visualised by TEM were electron-transparent since they comprised lipids containing saturated fatty acids that did not react with osmium tetroxide. The globules appeared to be bounded by a single membrane.
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Abbreviations
- TAG :
-
triacylglycerol
- TEM :
-
transmission electron microscopy
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Packter, N.M., Olukoshi, E.R. Ultrastructural studies of neutral lipid localisation inStreptomyces . Arch. Microbiol. 164, 420–427 (1995). https://doi.org/10.1007/BF02529740
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DOI: https://doi.org/10.1007/BF02529740