Abstract
The preferred positions for meiotic double-strand breakage were mapped onSaccharomyces cerevisiae chromosomes I and VI, and on a number of yeast artificial chromosomes carrying human DNA inserts. Each chromosome had strong and weak double-strand break (DSB) sites. On average one DSB-prone region was detected by pulsed-field gel electrophoresis per 25 kb of DNA, but each chromosome had a unique distribution of DSB sites. There were no preferred meiotic DSB sites near the telomeres. DSB-prone regions were associated with all of the known “hot spots” for meiotic recombination on chromosomes I, III and VI.
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Edited by: J.-L. Rossignol
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Klein, S., Zenvirth, D., Dror, V. et al. Patterns of meiotic double-strand breakage on native and artificial yeast chromosomes. Chromosoma 105, 276–284 (1996). https://doi.org/10.1007/BF02524645
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DOI: https://doi.org/10.1007/BF02524645