Abstract
A method for estimating free gossypol (FG) has been developed that decreases sample-determination time from over 2 h to about 25 min per sample. With auto pipetters and bottle-to dispensers, six sample determinations can be completed in approximately 50 min. The method consisted of adding water and acetone separately to a fixed sample weight, mixing, filtering, diluting with 65% acetone, and reading absorbance on a spectrophotometer. Absorbance was plotted against the official American Oil Chemists’ Society’s FG method for samples that contained FG between 0.02 and 0.9%. Quadratic least squares regression for 31 samples had a correlation coefficient ofr 2=0.986 and a standard error of estimated FG of 0.032%.
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Hron, R.J., Kuk, M.S. & Wan, P.J. Quick method for estimating free gossypol in cottonseed, meats, collets, and extracted meals. J Am Oil Chem Soc 73, 199–202 (1996). https://doi.org/10.1007/BF02523895
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DOI: https://doi.org/10.1007/BF02523895