Abstract
Dielectrophoresis has been used to enrich selected cell subpopulations in a mixed cell population by exploiting differential dielectric properties. Six-fold enrichment of stem cells expressing the CD34+ antigen has been achieved for bone marrow samples and peripheral blood, without the requirment for initial chemical treatment associated with immunoadsorption techniques.
Article PDF
Avoid common mistakes on your manuscript.
References
Becker, F. F., Wang, X-B., Huang, Y., Pethig, R., Vykoukal, J., andGascoyne, P. R. C. (1994a): ‘Separation of human breast cancer cells from blood by differential dielectric affinity’.Proc. Nat. Acad. Sci. USA
Becker, F. F., Wang, X.-B., Huang, Y., Pethig, R. Vykoukal, J., andGascoyne, P. R. C. (1994b): ‘The removal of human leukaemia cells from blood using interdigitated microelectrodes’.J. Phys. D. Appl. Phys.
Bendeer, J. G., Unverzagt, K. L., Walker, D. E., Lee, W., Van Epps, D. E., Smith, D. H., Stewart, C. C., andBik, To, L. (1991): ‘Identification and comparison of CD34 positive cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry,’Blood,77, pp. 2591–2596
Gascoyne, P. R. C., Huang, Y., Pethig, R., Vykoukal, J., andBecker, F. F. (1992): ‘Dielectrophoretic spearation of mammalian cells studied by computerized image analysis,’Meas. Sci. Technol.,3, pp. 439–445
Gascoyne, P. R. C., Pethig, R., Burt, J. P. H., andBecker, F. F. (1993): ‘Membrane changes accompanying the induced differentiation of Friend, murine erythroleukaemia cells studied by dielectrophoresis,’Biochim. Biophys. Acta,1149, pp. 119–126
Gascoyne, P. R. C., Noshari, J., Becker, F. F., andPethig, R. (1994): ‘Use of Dielectrophoretic Collection spectra for characterising differences between normal and cancerous cells,’IEEE Trans,IA-30, pp. 829–834
Markx, G. H., Huang, Y., Zhou, X.-F., andPethig, R. (1994a): ‘Dielectrophoretic characterization and separation of micro-organisms,’Microbiol.,140, pp. 585–591
Markx, G. H., Talary, M. S., andPethig, R. (1994b): ‘Separation of viable and non-viable yeart using dielectrophoresis,’J. Biotechnol.,32, pp. 29–37
Pohl, H. A. (1978): ‘Dielectrophoresis’ (Cambridge University Press, Cambridge, UK)
Price, J. A. R., Burt, J. P. H., andPethig, R. (1988): ‘Applications of a new optical technique for measuring the dielectrophoretic behaviour of micro-organisms,’Biochim. Biophys. Acta,964, pp. 221–230
Talary, M. S., andPethig, R. (1994): ‘Optical technique for measuring the positive and negative dielectrophoretic behavior of cells and colloidal suspensions,’IEE Proc. Sci. Meas., Technol.,141, pp. 395–399
Wang, X.-B., Huang, Y. Hölzel, R., Burt, J. P. H., andPethig, R. (1993): ‘Theoretical and experimental investigations of the dielectric, dielectrophoretic and electrorotational behaviour of colloidal, particles,’J. Phys. D. Appl. Phys.,26, pp. 312–322
Wang, X.-B., Huang, Y., Gascoyne, P. R. C., Becker, F. F., Hölzel, R., andPethig, R. (1994): ‘Changes in Friend murine erythroleukaemia cell membranes during induced differentiation determined by electrorotation,’Biochim. Biophys. Acta,1193, pp. 330–344
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Talary, M.S., Mills, K.I., Hoy, T. et al. Dielectrophoretic separation and enrichment of CD34+cell subpopulation from bone marrow and peripheral blood stem cells. Med. Biol. Eng. Comput. 33, 235–237 (1995). https://doi.org/10.1007/BF02523050
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02523050