Abstract
The changes in the triacylglycerol (TAG) composition of colostrum fat of three cows were studied. In addition to the determination of fatty acid composition by gas chromatography, the distribution of TAG according to the acyl carbon number (ACN) and molecular weight was analyzed utilizing both supercritical fluid chromatography (SFC) and ammonia negative-ion chemical ionization mass spectrometry (MS). Colostrum TAG contained substantially less stearic and oleic acids and more myristic and palmitic acids than the normal Finnish milk fat. The major trends in the changes of fatty acids and TAG were similar for each cow, although clear differences between individuals were found. During the first week of parturition, the proportions of short-chain fatty acids (C4–C10) typically increased as well as those of stearic and oleic acids, whereas the relative amounts of C12–C16 acids decreased, especially those of myristic and palmitic acids. Distinct changes occurred also in TAG distributions: the proportions of molecules with ACN 38–40 increased and those with ACN 44–48 decreased. Although there were distinct differences between individuals shortly after delivery, both the fatty acid compositions and TAG distributions of the milk samples of the cows started to resemble each other after one week. The theoretical profiles of colostrum TAG calculated based on the fatty acid compositions differed clearly from the ACN distributions analyzed by SFC and MS. Thus, the analysis of TAG is essential, because the changes in molecular species composition of colostrum TAG cannot be estimated according to the fatty acid analysis alone.
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Abbreviations
- ACN:
-
acyl carbon number
- FID:
-
flame-ionization detector
- GC:
-
gas chromatography
- HPLC:
-
high-performance liquid chromatography
- MS:
-
mass spectrometry
- SFC:
-
supercritical fluid chromatography
- TAG:
-
triacylglycerols
References
Lund, P. (1988) Analysis of Butterfat Triglycerides by Capillary Gas Chromatography,Milchwissenschaft 43, 159–161.
Maniongui, C., Gresti, J., Bugaut, M., Gauthier, S., and Bezard, J. (1991) Determination of Bovine Butterfat Triacylglycerols by Reversed-Phase Liquid Chromatography and Gas Chromatography,J. Chromatogr. 543, 81–103.
Geeraert, E., and Sandra, P. (1985) Capillary GC of Triglycerides in Fats and Oils Using a High Temperature Phenylmethylsilicone Stationary Phase, Part I,J. High Resolut. Chromatogr. Chromatogr. Commun., 8, 415–422.
Myher, J.J., Kuksis, A., Marai, L., and Sandra, P. (1988) Identification of the More Complex Triacylglycerols in Bovine Milk Fat by Gas Chromatography-Mass Spectrometry Using Polar Capillary Columns,J. Chromatogr. 452, 93–118.
Kalo, P., and Kemppinen, A. (1993) Mass Spectrometric Identification of Triacylglycerols of Enzymatically Modified Butterfat Separated on a Polarizable Phenylmethylsilicone Column,J. Am. Oil Chem. Soc. 70, 1209–1217.
Kuksis, A., Marai, L., and Myher, J.J. (1991) Reversed-Phase Liquid Chromatography-Mass Spectrometry of Complex Mixtures of Natural Triacylglycerols with Chloride-Attachment Negative Chemical Ionization,J. Chromatogr. 588, 73–87.
Laakso, P.H., Nurmela, K.V.V., and Homer, D.R. (1992) Composition of the Triacylglycerols of Butterfat and Its Fractions Obtained by an Industrial Melt Crystallization Process,J. Agric. Food Chem. 40, 2472–2482.
Laakso, P., and Kallio, H. (1993) Triacylglycerols of Winter Butterfat Containing Configurational Isomers of Monoenoic Fatty Acyl Residues. I. Disaturated Monoenoic Triacylglycerols.J. Am. Oil Chem. Soc. 70, 1161–1171.
Marai, L., Kuksis, A., and Myher, J.J. (1994) Reversed-Phase Liquid Chromatography-Mass Spectrometry of the Uncommon Triacylglycerol Structures Generated by Randomization of Butteroil,J. Chromatogr. A672, 87–99.
Christie, W.W. (1991) Recent Developments in High-Performance Liquid and Gas Chromatography of Lipids,Rev. Fr. Corps Gras 38, 155–160.
Brühl, L., Schulte, E., and Thier, H.-P. (1993) Fraktionierung der Triglyceride von Muttermilch durch HPLC an einer Silberionensäule und an RP-18-Material mit dem Lichstreudetektor,Fat Sci. Technol. 95, 370–376.
Winter, C.H., Hoving, E.B., and Muskiet, F.A.J. (1993) Fatty Acid Composition of Human Milk Triglyceride Species. Possible Consequences for Optimal Structures of Infant Formula Triglycerides,J. Chromatogr. 616, 9–24.
Manninen, P., Laakso, P., and Kallio, H. (1995) Method for Characterization of Triacylglycerols and Fat-Soluble Vitamins in Edible Oils and Fats by Supercritical Fluid Chromatography,J. Am. Oil Chem. Soc. 72, 1001–1008.
Murata, T., and Takahashi, S. (1973) Analysis of Triglyceride Mixtures by Gas Chromatography-Mass Spectrometry,Anal. Chem. 45, 1816–1823.
Schulte, E., Höhn, M., and Rapp, U. (1981) Mass Spectrometric Determination of Triglyceride Patterns of Fats by the Direct Chemical Ionization Technique (DCI),Fresenius Z. Anal. Chem. 307, 115–119.
Spanos, G.A., Schwartz, S.J., van Breemen, R.B., and Huang, C.-H. (1995) High-Performance Liquid Chromatography with Light-Scattering Detection and Desorption Chemical-Ionization Tandem Mass Spectrometry of Milk Fat Triacylglycerols,Lipids 30, 85–90.
Kallio, H., and Currie, G. (1993) Analysis of Low Erucic Acid Turnip Rapeseed Oil (Brassica campestris) by Negative Ion Chemical Ionization Tandem Mass Spectrometry. A Method Giving Information on the Fatty Acid Composition in Positionssn-2 andsn-1/3 of Triacylglycerols.Lipids 28, 207–215.
Laakso, P., and Kallio, H. (1996) Optimization of the Mass Spectrometric Analysis of Triacylglycerols Using Negative-Ion Chemical Ionization with Ammonia,Lipids 31, 33–42.
Stull, J.W., Brown, W.H., Valdez, C., and Tucker, H. (1966) Fatty Acid Composition of Milk. III. Variation with Stage of Lactation,J. Dairy Sci. 49, 1401–1405.
Senft, B., and Klobasa, F. (1970) Untersuchungen über das Fettsäurespektrum in der Kolostralmilch von Kühen,Milchwissenschaft 25, 391–394.
Parodi, P.W. (1972) Observations on the Variation in Fatty Acid Composition of Milkfat,Aust. J. Dairy Technol. 27, 90–94.
Baše, J., Škarda, J., Urbanová, E., and Bílek, J. (1983) The Proportion of Fatty Acids in Mammary Secretion of Cows Lactating After Calving and Hormonal Induction of Lactation,Physiol. Bohemoslov, 32, 155–161.
Banerjee, R., Bandyopadhyay, C., and Subrahmanyam, V.V.R. (1991) Composition of Cow's Milk-Fat During Transition from Colostrum to Normal. Part II: Changes in Fatty Acids and Glycerides.Indian J. Dairy Sci. 44, 66–70.
Christie, W.W. (1979) The Effects of Diet and Other Factors on the Lipid Composition of Ruminant Tissues and Milk,Prog. Lipid Res. 17, 245–277.
Hawke, J.C., and Taylor, M.W. (1995) Influence of Nutritional Factors on the Yield, Composition and Physical Properties of Milk Fat, inAdvanced Dairy Chemistry. 2: Lipids, 2nd edn. (Fox, P.F., ed.) pp. 37–88, Chapman and Hall, London.
Kuksis, A., McCarthy, M.J., and Beveridge, J.M.R. (1963) Quantitative Gas-Liquid Chromatographic Analysis of Butter-fat Triglycerides.J. Am. Oil Chem. Soc. 40, 530–535.
Kuksis, A., McCarthy, M.J., and Beveridge, J.M.R. (1964) Triglyceride Composition of Native and Rearranged Butter and Coconut Oils.J. Am. Oil Chem. Soc. 41, 201–205.
Gresti, J., Bugaut, M., Maniongui, C., and Bezard, J. (1993) Composition of Molecular Species of Triacylglycerols in Bovine Milk Fat,J. Dairy Sci. 76, 1850–1869.
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Laakso, P., Manninen, P., Mäkinen, J. et al. Postparturition changes in the triacylglycerols of cow colostrum. Lipids 31, 937–943 (1996). https://doi.org/10.1007/BF02522686
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DOI: https://doi.org/10.1007/BF02522686