Abstract
The purpose of this study was to find a possible explanation for the coexistence of large and small purple sulfur bacteria in natural habitats. Experiments were carried out withChromatium vinosum SMG 185 andChromatium weissei SMG 171, grown in both batch and continuous cultures. The data may be summarized as follows: (a) In continuous light, with sulfide as growth rate-limiting substrate, the specific growth rate ofChr. vinosum exceeds that ofChr. weissei regardless of the sulfide concentration employed. Consequently,Chr. weissei is unable to compete successfully and is washed out in continuous cultures. (b) With intermittant light-dark illumination, the organisms showed balanced coexistence when grown in continuous cultures. The “steady-state” abundance ofChr. vinosum was found to be positively related to the length of the light period, and that ofChr. weissei to the length of the dark period. (c) Sulfide added during darkness is rapidly oxidized on subsequent illumination, resulting in the intracellular storage of reserve substances, which are later utilized for growth. The rate of sulfide oxidation/mg cell N/hr was found to be over twice as high inChr. weissei as inChr. vinosum. The observed coexistence may be explained as follows. In the light, with both strains growing, most of the sulfide will be oxidized byChr. vinosum [see (a)]. In the dark, sulfide accumulates. On illumination, the greater part of the accumulated sulfide will be oxidized byChr. weissei [see (c)]. A changed light-dark regimen should then have the effect as observed [see (b)]. These observations suggest that intermittant illumination may, at least in part explain the observed coexistence of both types of purple sulfur bacteria in nature.
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References
Andrews, J. F. 1968. A mathematical model for the continuous culture of microorganisms utilizing inhibitory substrates.Biotechnol. Bioeng. 10: 707–723.
van Gemerden, H. 1968. Growth measurements ofChromatium cultures.Arch. Mikrobiol. 64: 103–110.
van Gemerden, H. 1968 Utilization of reducing power in growing cultures ofChromatium.Arch. Mikrobiol 64: 111–117.
van Gemerden, H., and H. W. Jannasch. 1971. Continuous culture of Thiorhodaceae. Sulfide and sulfur limited growth ofChromatium vinosum.Arch. Mikrobiol. 79: 345–353.
Hansen, T. A., and H. van Gemerden. 1972. Sulfide utilization by purple nonsulfur bacteria.Arch. Mikrobiol. 86: 49–56.
Hansen, T. A., and H. Veldkamp. 1973.Rhodopseudomonas sulfidophila, nov. spec., a new species of the purple nonsulfur bacteria.Arch. Mikrobiol. 92: 45–58.
Hirsch, P. 1969. Growth and structure of the purple sulfur bacteriumThiopedia from natural sources and laboratory cultures.Bact. Proc. 1969: 32
Kaiser, P. 1966. Ecologie des bactéries photosynthétiques.Rev. Ecol. Biol. Sol. 3: 409–472.
Kondrat'eva, E. N. 1965.Photosynthetic Bacteria. (Translated from Russian.) Israel Program for Scientific Translations, Jerusalem.
Pfenning, N. 1961. Eine vollsynthetische Nährlösung zur selektiven Anreicherung einiger Schwefelpurperbakterien.Naturwissenschaften 48: 136.
Pfenning, N. 1962. Beobachtungen über das Schwärmen vonChromatium okenii.Arch. Mikrobiol. 42: 90–95.
Pfennig, N. 1965. Anreicherungskulturen für rote und grüne Schwefelbakterien.Zentr. Bakteriol. Parasitenk. Abt. I. Suppl. 1: 179–189.
Pfennig, N. 1967. Photosynthetic bacteria.Ann. Rev. Microbiol. 21: 285–324.
Pfennig, N., and H. G. Trüper. 1969.Phototrophic Bacteria. G.S.F.-Bericht M32.
Schlegel, H. G., and N. Pfennig. 1961. Die Anreicherungskultur einiger Schwefelpurperbakterien.Arch. Mikrobiol. 38: 1–39.
Sirevåg, R., and J. G. Ormerod. 1970. Carbon dioxide fixation in green sulphur bacteria.Biochem. J. 120: 399–408.
Thiele, H. H. 1968. Die Verwertung einfacher organischer Substrate durch Thiorhodaceae.Arch. Mikrobiol. 60: 124–138
Trüper, H. G., and H. G. Schlegel. 1964. Sulphur metabolism in Thiorhodaceae. I. Quantitative measurements on growing cells ofChromatium okenii.Ant. Leeuwenhoek 30: 225–238.
Trüper, H. G. 1968.Ectothiorhodospira mobilis Pelsh, a photosynthetic sulfur bacterium deposting sulfur outside the cells.J. Bacteriol. 95: 1910–1920.
Trüper, H. G., and S. Genovese. 1968. Characterization of photosynthetic sulfur bacteria causing red water in lake Faro (Messina, Sicily).Limnol. Oceanogr. 13: 225–232.
Trüper, H. G., and H. W. Jannasch 1968.Chromatium buderi Nov. Spec., eine neue Art der “grozen” ThiorhodaceaeArch.Mikrobiol. 61: 363–372.
Van Niel, C. B. 1936. On the metabolism of the Thiorhodaceae.Arch. Mikrobiol. 7: 323–358.
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van Gemerden, H. Coexistence of organisms competing for the same substrate: An example among the purple sulfur bacteria. Microb Ecol 1, 104–119 (1974). https://doi.org/10.1007/BF02512382
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DOI: https://doi.org/10.1007/BF02512382