Summary
A specific and sensitive direct-injection high performance liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry (HPLC-APCI-MS-MS) method has been developed for the rapid identification and quantitative determination of citalopram, fluvoxamine, and paroxetine in human plasma. After dilution with 0.1% formic acid, plasma samples were injected into the LC-MS-MS system. Proteins and other large biomolecules were removed during an on-line sample cleanup step. The inter- and intra-assay coefficients of variation for all compounds were <11%. The total analysis time was 6 min per sample. The proposed method permits direct analysis of plasma samples without time-consuming sample preparation.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Mourilhe, P.; Stokes, P.E.Drug Saf. 1998,18, 57–82.
Gunasekara, N.S.; Noble, S.; Benfield, P.Drugs 1998,55, 85–120.
Preskorn, S.H.J. Clin. Psychiatry 1994,55, Suppl. A, 6–22.
Maurer, H.H.; Bickeboeller-Friedrich, J.J. Anal. Toxicol. 2000,24, 340–347.
Eap, C.B.; Bouchoux, G.; Amey, M.; Cochard, N.; Savary, L.; Baumann, P.J. Chromatogr. Sci. 1998,36, 365–371.
Fu, K.; Konrad, R.J.; Hardy, R.W.; Brissie, R.M.; Robinson, C.A.J. Anal. Toxicol. 2000,24, 648–650.
Lacassie, E.; Gaulier, J.M.; Marquet, P.; Rabatel, J.F.; Lachatre, G.J. Chromatogr. B 2000,742, 229–238.
Jourdil, N.H.; Fontanille, P.D.; Bessard, G.M.Clin. Chem. 1997,43, 2209–2210.
Molander, P.; Thomassen, A.; Kristoffersen, L.; Greibrokk, T.; Lundanes, E.J. Chromatogr. B 2002,766, 77–87.
Tournel, G.; Houdret, N.; Hedouin, V.; Deveau, M.; Gosset D.; Lhermitte, M.J. Chromatogr. B 2001,761, 147–158.
Ohman, D.; Carlsson, B.; Norlander, B.J. Chromatogr. B 2001,753, 365–373.
Palego, L.; Marazziti, D.; Biondi, L.; Giannaccini, G.; Sarno, N.; Armani, A.; Lucacchini, A.; Cassano, G.B.; Dell'Osso L.Ther. Drug Monit. 2000,22, 190–194.
Zheng, Z.; Jamour, M.; Klotz, U.Ther. Drug. Monit. 2000,22, 219–224.
Kristoffersen, L.; Bugge, A.; Lundanes, E.; Slordal, L.J. Chromatogr. B 1999,734, 229–246.
Olesen, O.V.; Linnet, K.J. Chromatogr. B 1996,675, 83–88.
Belmadani, A.; Combourieu, I.; Bonini, M.; Creppy, E.E.Hum. Exp. Toxicol. 1995,14, 34–37.
Knoeller, J.; Vogt-Schenkel, R.; Brett, M.A.J. Pharm. Biomed. Anal. 1995,13, 635–638.
Lopez-Calull, C.; Dominguez, N.J. Chromatogr. B 1999,724, 393–398.
Shin, J.G.; Kim, K.A.; Yoon, Y.R.; Cha, I.J.; Kim, Y.H.; Shin, S.G.J. Chromatogr. B 1998,713, 452–456.
Carlsson, B.; Norlander, B.J. Chromatogr. B 1997,702, 234–239.
Matsui, E.; Hoshino, M.; Matsui, A.; Okahira, A.J. Chromatogr. B 1995,668, 299–307.
Rochat, B.; Amey, M.; Baumann, P.Ther. Drug Monit. 1995,17, 273–279.
Pullen, R.H.; Fatmi, A.A.J. Chromatogr. 1992,574, 101–107.
Ayrton, J.; Dear, G.J.; Leavens, W.J.; Mallett, D.N.; Plumb, R.S.Rapid. Commun. Mass. Spectrom. 1997,11, 1953–1958.
Ayrton, J.; Dear, G.J.; Leavens, W.J.; Mallett, D.N.; Plumb, R.S.J. Chromatogr. A 1998,828, 199–207.
Quinn, H.M.; Takarewski, J.J. International Patent Number WO 97/16724.
Matuszewski, B.K.; Constanzer, M.L.; Chavez-Eng C.M.Anal. Chem. 1998,70, 882–889.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kollroser, M., Schober, C. An on-line solid phase extraction—Liquid chromatography—Tandem mass spectrometry method for the analysis of citalopram, fluvoxamine, and paroxetine in human plasma. Chromatographia 57, 133–138 (2003). https://doi.org/10.1007/BF02491705
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02491705