Summary
A simple and sensitive method is presented for determination of styrene, toluene, ethylbenzene, isopropylbenzene andn-propylbenzene in human body fluids by capillary gas chromatography (GC) with cryogenic oven trapping. After heating a blood or urine sample containing each compound andp-diethylbenzene (internal standard, IS) in a 7.0-mL vial at 60°C for 20 min, 5 mL of headspace vapor was drawn into a glass syringe and injected into a GC. All vapor was introduced into an Rtx-Volatile middle bore capillary column in splitless mode at oven temperature of 20°C to trap entire analytes, and the oven temperature then programmed to 280°C for GC measurements by flame ionization detection. The present conditions gave sharp peaks of each compound and IS, and low background noises for whole blood or urine samples.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Pratt-Johnson, J.A.Can. Med. Assoc. J. 1964,90, 975–977.
Waxweiler, R.J.; Stringer, W.; Wagoner, J.K.; Jones, J.Ann. N. Y. Acad. Sci. 1978,271, 40–48.
Lorimer, W.V.; Lilis, R.; Nicholson, W.J.; Anderson, H.; Fischbein, A.; Daum, S.; Rom, W.; Rice, C.; Selikoff, I.J.Environ. Health Perspect. 1976,17, 171–181.
Lindström, K.; Härkönen, H.; Hernberg, S.Scand. J. Work Environ. Health 1976,3, 129–139.
Lilis, R.; Lorimer, W.V.; Diamond, S.; Selikoff, I.J.Environ. Res. 1978,15, 133–138.
Vainio, H.Chem. Biol. Interact. 1978,22, 117–124.
Härkönen, H.Scand. J. Work Environ. Health 1978,4, 104–113.
Ponomarkov, V.Scand. J. Work Environ. Health 1978,4, 127–135.
Rosén, I.; Haeger-Aronsen, B.; Rehnström, S.; Welinder, H.Scand. J. Work Environ. Health 1978,4, 184–194.
Nicholson, W.J.; Selikoff, I.J.; Seidman H.Scand. J. Work Environ. Health 1978,4, 247–252.
Engström, J.; Åstrand, I.; Wigaeus, E.;Scand. J. Work Environ. Health 1978,4, 324–329.
Cherry, N.; Waldron, H.A.; Wells, G.G.; Wilkinson, R.T.; Wilson, H.K.; Jones, S.Br. J. Ind. Med. 1980,37, 234–240.
Bond, J.A.Crit. Rev. Toxicol. 1989,19, 227–249.
Naito, H.Posoning of Industrial Products, Gases Pesticides, Drugs, and Natural Toxins. Nankodo, Tokyo,1991.
Baker, E.L.; Fine, L.J.J. Occup. Med. 1986,28, 126–129.
Wallace, L.; Pellizzari, E.; Wendel, C.Indoor Air 1991,4, 465–477.
Molhave, L.; Bach, B.; Pedersen, O.F.Environ. Int. 1986,12, 167–175.
Hodgson, A.T.; Girman, J.R. InDesign and Protocol for Monitoring Indoor Air Quality: Nagda, N.L.; Harper, J.P., Eds. American Society for Testing and Materials, Philadelphia,1989.
Otto, D.A.; Hudnell, H.K.; House, D.E.; Molhave, L.; Counts, W.Arch. Environ. Health 1992,47, 23–30.
Varner, S.L.; Breder, C.V.; Fazio, T.J. Assoc. Off. Anal. Chem. 1983,66, 1067–1073.
Nerin, C.; Rubio, C.; Cacho, J.; Salafranca, J.Food. Addit. Contam. 1998,15, 346–354.
Byrd, G.D.; Fowler, K.W.; Hicks, R.D.; Lovette, M.E.; Borgerding, M.F.J. Chromatogr. 1990,503, 359–368.
Foerster, E.H.; Garriott, J.C.J. Anal. Toxicol. 1981,5, 241–244.
Pantarotto, C.; Fanelli, R.; Belletti, I.; Bidoli, F.Anal. Biochem. 1980,105, 340–347.
Ishizawa, F.; Misawa S.J. Forensic Sci. Soc. 1990,30, 201–209.
Watanabe, K.; Seno, H.; Ishii, A.; Suzuki, O.; Kumazawa, T.Anal. Chem. 1997,69, 5178–5181.
Lee, X.-P.; Kumazawa, T.; Sato, K.; Watanabe, K.; Seno, H.; Suzuki, O.Analyst 1998,123, 147–150.
Hattori, H.; Iwai, M.; Kurono, S.; Yamada, T.; Watanabe-Suzuki, K.; Ishii, A.; Seno, H.; Suzuki, O.J. Chromatogr. B 1998,718, 285–289.
Watanabe-Suzuki, K.; Seno, H.; Ishii, A.; Kumazawa, T.; Suzuki, O.J. Chromatogr. B 1999,727, 89–94.
Watanabe-Suzuki, K.; Ishii, A.; Lee, X.-P.; Suzuki, O.Jpn. J. Forensic Toxicol. 2000,18, 201–209.
Christmore, D.S.; Kelly, R.C.; Doshier, L.A.J. Forensic Sci. 1984,29, 1038–1044.
Seto, Y.J. Chromatogr. A 1994,674, 25–62.
Medinsky, M.A.; Klaassen, C.D. InCasarett and Doull's Toxicology, 5th Ed: Klaassen, C.D., Ed, McGraw-Hill, New York,1996.
Steele, D.H.; Thornburg, M.J.; Stanley, J.S.; Miller, R.R.; Brooke, R.; Cushman, J.R.; Cruzan, G.J. Agric. Food Chem. 1994,42, 1661–1665.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Watanabe-Suzuki, K., Ishii, A., Seno, H. et al. Sensitive determination of styrene and related compounds in human body fluids by headspace capillary gas chromatography with cryogenic oven trapping. Chromatographia 54, 507–510 (2001). https://doi.org/10.1007/BF02491208
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02491208